Plasmid vectors harboring cellular promoters can induce prolonged gene expression in hematopoietic and mesenchymal progenitor cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Byun, HM | - |
dc.contributor.author | Suh, DC | - |
dc.contributor.author | Jeong, YS | - |
dc.contributor.author | Wee, HS | - |
dc.contributor.author | Kim, JM | - |
dc.contributor.author | Kim, WK | - |
dc.contributor.author | Ko, JJ | - |
dc.contributor.author | Kim, JS | - |
dc.contributor.author | Lee, YB | - |
dc.contributor.author | Oh, YK | - |
dc.date.available | 2021-02-22T15:46:35Z | - |
dc.date.issued | 2005-07 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15489 | - |
dc.description.abstract | Although prolonged transgene expression in progenitor cells might be desirable for modified cell therapy, the viral promoter-based expression vector tends to promote transgene expression only for a limited period. Here, we examined the ability of cellular promoters from elongation factor-1 alpha (EF-1 alpha) and ubiquitin C to drive gene expression in hematopoietic TF-1 and mesenchymal progenitor cells. We compared the expression levels and duration of a model gene, interleukin-2, generated by the cellular promoters to those by the cytomegalovirus (CMV) promoter. The EF-1 alpha and ubiquitin C promoters drove prolonged gene expression in hematopoietic TF-1 and mesenchymal progenitor cells, whereas the CMV promoter did not. At day 7 after transfection in TF-1 cells, the mRNA expression levels of interleukin-2 driven by the EF-1 alpha and ubiquitin C promoters were 118- and 56-fold higher, respectively, than those driven by the CMV promoter. Similarly, in mesenchymal progenitor cells, the expression levels of interleukin-2 driven by the EF-1 alpha and ubiquitin C promoters were 98- and 20-fold higher, respectively, than that driven by the CMV promoter-encoding plasmid. Moreover, the ubiquitin C promoter directed higher levels of green fluorescence protein expression in mesenchymal progenitor cells than did the CMV promoter. These results indicate that the use of cellular promoters such as those for EF-1 alpha and ubiquitin C might direct prolonged gene expression in hematopoietic and mesenchymal progenitor cells. (C) 2005 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Plasmid vectors harboring cellular promoters can induce prolonged gene expression in hematopoietic and mesenchymal progenitor cells | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.bbrc.2005.04.155 | - |
dc.identifier.scopusid | 2-s2.0-21144457927 | - |
dc.identifier.wosid | 000229572300030 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.332, no.2, pp 518 - 523 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 332 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 518 | - |
dc.citation.endPage | 523 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | UBIQUITIN-C PROMOTER | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | TRANSGENE EXPRESSION | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CD34(+) | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordAuthor | promoters | - |
dc.subject.keywordAuthor | ubiquitin C | - |
dc.subject.keywordAuthor | elongation factor-1 alpha | - |
dc.subject.keywordAuthor | hematopoietic cells | - |
dc.subject.keywordAuthor | mesenchymal progenitor cells | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.