Characterization of a second gene encoding gamma-glutamyl transpeptidase from Schizosaccharomyces pombe
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, HJ | - |
dc.contributor.author | Moon, JS | - |
dc.contributor.author | Kim, HG | - |
dc.contributor.author | Kim, IH | - |
dc.contributor.author | Kim, K | - |
dc.contributor.author | Park, EH | - |
dc.contributor.author | Lim, CJ | - |
dc.date.available | 2021-02-22T15:47:08Z | - |
dc.date.issued | 2005-03 | - |
dc.identifier.issn | 0008-4166 | - |
dc.identifier.issn | 1480-3275 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15531 | - |
dc.description.abstract | The first gene encoding gamma-glutamyl transpeptidase (GGTI) of the fission yeast has previously been characterized, and its expression was found to be regulated by various oxidative stress-inducing agents. In this work, a second gene, encoding GGTII, was cloned and characterized from the fission yeast Schizosaccharomyces pombe. The structural gene encoding GGTII was amplified from the genomic DNA of the fission yeast and ligated into the shuttle vector pRS316 to generate the recombinant plasmid pPHJ02. The determined sequence contains 3040 bp and is able to encode the putative 611 amino acid sequence of GGTII, which resembles the counterparts of Saccharomyces cerevisiae, Homo sapiens, Rattus norvegicus, and Escherichia coli. The DNA sequence also contains 940-bp upstream and 289-bp downstream regions of the GGTII gene. The Schizosaccharomyces pombe cells harboring plasmid pPHJ02 showed about 4-fold higher GGT activity in the exponential phase than the cells harboring the vector only, indicating that the cloned GGTII gene is functional. The S. pombe cells containing the cloned GGTII gene were found to contain higher levels of both intracellular glutathione (GSH) content and GSH uptake. The S. pombe cells harboring plasmid pPHJ02 showed increased survival on solid media containing hydrogen peroxide, diethylmaleate, aluminum chloride, cadmium chloride, or mercuric chloride. The GGTII mRNA level was significantly elevated by treatment with GSH-depleting diethylmaleate. These results imply that the S. pombe GGTII gene produces functional GGTII protein and is involved in the response to oxidative stresses in S. pombe cells. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | CANADIAN SCIENCE PUBLISHING | - |
dc.title | Characterization of a second gene encoding gamma-glutamyl transpeptidase from Schizosaccharomyces pombe | - |
dc.type | Article | - |
dc.publisher.location | 캐나다 | - |
dc.identifier.doi | 10.1139/W04-137 | - |
dc.identifier.scopusid | 2-s2.0-22844448549 | - |
dc.identifier.wosid | 000230140900010 | - |
dc.identifier.bibliographicCitation | CANADIAN JOURNAL OF MICROBIOLOGY, v.51, no.3, pp 269 - 275 | - |
dc.citation.title | CANADIAN JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 51 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 269 | - |
dc.citation.endPage | 275 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | GLUTATHIONE | - |
dc.subject.keywordPlus | TRANSFERASE | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | fission yeast | - |
dc.subject.keywordAuthor | genomic DNA | - |
dc.subject.keywordAuthor | gamma-glutamyl transpeptidase | - |
dc.subject.keywordAuthor | regulation | - |
dc.subject.keywordAuthor | Schizosaccharomyces pombe | - |
dc.subject.keywordAuthor | stress response | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.