Protein profiling and identification of modulators regulated by human papillomavirus 16 E7 oncogene in HaCaT keratinocytes by proteomics
DC Field | Value | Language |
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dc.contributor.author | Lee K.-A. | - |
dc.contributor.author | Kang J.-W. | - |
dc.contributor.author | Shim J.-H. | - |
dc.contributor.author | Chang W.K. | - |
dc.contributor.author | Sung G.P. | - |
dc.contributor.author | Hee G.L. | - |
dc.contributor.author | Paik S.-G. | - |
dc.contributor.author | Lim J.-S. | - |
dc.contributor.author | Yoon D.-Y. | - |
dc.date.available | 2021-02-22T16:01:04Z | - |
dc.date.issued | 2005-10 | - |
dc.identifier.issn | 0090-8258 | - |
dc.identifier.issn | 1095-6859 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15765 | - |
dc.description.abstract | Objectives. Viral oncogenes E6 and E7 are selectively retained and expressed in carcinoma cells infected with human papillomavirus type 16 and cooperated with each other in immortalization and transformation of primary keratinocytes. This study was performed to identify proteins to be bound or modulated by high risk HPV E7 oncogene by using a proteomics. Methods. HaCaT normal keratinocyte was prepared to establish a stable cell line expressing E7. The E7-affinity column was also prepared to obtain E7-interacting proteins. In order to search the target molecules modulated by E7 expression, we used 2-dimensional electrophoresis and matrix-assisted laser desorption/ionization time of flight (MALDI/TOF) mass spectrometry. Pull down assay was also performed in order to confirm the E7-interacting proteins. Results. We identified 28 spots that are modulated by E7 in HaCaT/E7 using 2-dimensional electrophoresis (2-DE) and MALDI/TOF mass spectrometry. Proteomics analyses showed that actin and leukocyte elastase inhibitor were down-regulated, whereas stress-induced phosphoprotein 1, CD2 binding protein 1, catalase, T-complex protein 1, Ku70-binding protein, heat shock 60 kDa protein 1, G1/S-specific cyclin E1 and peroxiredoxin 2 were up-regulated. Western blot revealed that heat shock 60 kDa protein, catalase and peroxiredoxin 2 were also up-regulated. Pull down assay also showed that leukocyte elastase inhibitor (LEI) and Ku70-binding protein were bound to the E7 oncoprotein. By using E7-affinity column and 2-DE/MALDI-TOF, 22 spots were found to interact with E7 recombinant protein. MG11-like proteins, livin inhibitor-of-apoptosis, protein serine kinase c17, CD2 binding protein 1, cyclin E1, TATA box binding protein-associated factor and uridine-cytidine kinase 2 were up-regulated by E7 oncogene and also bound to E7 oncoprotein. Conclusions. It is presumed that E7 can influence cell status by modulating the factors related to cell signaling, apoptosis and cell cycle regulation. © 2005 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | Protein profiling and identification of modulators regulated by human papillomavirus 16 E7 oncogene in HaCaT keratinocytes by proteomics | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.ygyno.2005.05.039 | - |
dc.identifier.scopusid | 2-s2.0-25144475834 | - |
dc.identifier.bibliographicCitation | Gynecologic Oncology, v.99, no.1, pp 142 - 152 | - |
dc.citation.title | Gynecologic Oncology | - |
dc.citation.volume | 99 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 142 | - |
dc.citation.endPage | 152 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | actin | - |
dc.subject.keywordPlus | binding protein | - |
dc.subject.keywordPlus | catalase | - |
dc.subject.keywordPlus | CD2 antigen | - |
dc.subject.keywordPlus | cd2 binding protein 1 | - |
dc.subject.keywordPlus | cyclin E | - |
dc.subject.keywordPlus | cyclin e1 | - |
dc.subject.keywordPlus | glycine cleavage system | - |
dc.subject.keywordPlus | heat shock protein 60 | - |
dc.subject.keywordPlus | Ku antigen | - |
dc.subject.keywordPlus | leukocyte elastase inhibitor | - |
dc.subject.keywordPlus | livin inhibitor of apoptosis protein | - |
dc.subject.keywordPlus | mg11 like protein | - |
dc.subject.keywordPlus | peroxiredoxin | - |
dc.subject.keywordPlus | phosphoprotein | - |
dc.subject.keywordPlus | protein derivative | - |
dc.subject.keywordPlus | protein E7 | - |
dc.subject.keywordPlus | protein serine kinase | - |
dc.subject.keywordPlus | protein serine kinase c17 | - |
dc.subject.keywordPlus | recombinant protein | - |
dc.subject.keywordPlus | TATA binding protein associated factor | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | uridine cytidine kinase 2 | - |
dc.subject.keywordPlus | uridine kinase | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell cycle | - |
dc.subject.keywordPlus | cell cycle G1 phase | - |
dc.subject.keywordPlus | cell cycle S phase | - |
dc.subject.keywordPlus | cell line | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | down regulation | - |
dc.subject.keywordPlus | embryo | - |
dc.subject.keywordPlus | gene expression | - |
dc.subject.keywordPlus | gene expression profiling | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | Human papillomavirus type 16 | - |
dc.subject.keywordPlus | keratinocyte | - |
dc.subject.keywordPlus | matrix assisted laser desorption ionization time of flight mass spectrometry | - |
dc.subject.keywordPlus | molecular weight | - |
dc.subject.keywordPlus | nucleotide sequence | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | protein analysis | - |
dc.subject.keywordPlus | protein interaction | - |
dc.subject.keywordPlus | proteomics | - |
dc.subject.keywordPlus | two dimensional electrophoresis | - |
dc.subject.keywordPlus | upregulation | - |
dc.subject.keywordPlus | virus expression | - |
dc.subject.keywordPlus | virus oncogene | - |
dc.subject.keywordPlus | Antigens, Nuclear | - |
dc.subject.keywordPlus | Cell Cycle Proteins | - |
dc.subject.keywordPlus | Cell Transformation, Viral | - |
dc.subject.keywordPlus | DNA-Binding Proteins | - |
dc.subject.keywordPlus | Down-Regulation | - |
dc.subject.keywordPlus | Electrophoresis, Gel, Two-Dimensional | - |
dc.subject.keywordPlus | Female | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Keratinocytes | - |
dc.subject.keywordPlus | Molecular Chaperones | - |
dc.subject.keywordPlus | Oncogene Proteins, Viral | - |
dc.subject.keywordPlus | Papillomaviridae | - |
dc.subject.keywordPlus | Papillomavirus Infections | - |
dc.subject.keywordPlus | Protein Binding | - |
dc.subject.keywordPlus | Proteomics | - |
dc.subject.keywordPlus | Serpins | - |
dc.subject.keywordPlus | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | - |
dc.subject.keywordPlus | Transfection | - |
dc.subject.keywordPlus | Up-Regulation | - |
dc.subject.keywordPlus | Uterine Cervical Neoplasms | - |
dc.subject.keywordAuthor | Affinity column | - |
dc.subject.keywordAuthor | E7 | - |
dc.subject.keywordAuthor | Human papillomavirus | - |
dc.subject.keywordAuthor | Proteomics | - |
dc.subject.keywordAuthor | Two-dimensional gel electrophoresis | - |
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