Regulation of enzymes involved in methionine biosynthesis in Corynebacterium glutamicum
DC Field | Value | Language |
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dc.contributor.author | Yeom, HJ | - |
dc.contributor.author | Hwang, BJ | - |
dc.contributor.author | Lee, MS | - |
dc.contributor.author | Kim, Y | - |
dc.contributor.author | Lee, HS | - |
dc.date.available | 2021-02-22T16:03:02Z | - |
dc.date.issued | 2004-04 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15892 | - |
dc.description.abstract | The regulatory mechanism of methionine biosynthesis in Corynebacterium glutamicum was analyzed at the protein and gene expression level. O-Acetylhomoserine sulfhydraylase (encoded by metY) was inhibited by 10 mM methionine to a residual activity of 10% level, whereas no such inhibition was found with cystathionine gamma-synthase (encoded by metB) and cystathioninebeta-lyase (encoded by metC). The enzymatic activity of homoserine acetyltransferase (encoded by metX) was repressed to a residual activity of 25% level by 10 mM methionine which was added to the growth medium. Cystathionine gamma-synthase and cystathionine beta-lyase were also repressed by 10 mM methionine, but only to a residual activity of 50-70% level. O-Acetylhomoserine sulfhydrylase was very sensitive to repression by 10 mM methionine, showing residual activity of 13%. In addition, homoserine acetyltransferase was also repressed by 10 mM cysteine to 50% of its original activity. No repression of the enzymes by S-adenosyl methionine was observed. The pattern of repression by methionine indicated that the metB and aecD genes might be regulated by a common mechanism, while the metA and metY genes are differently regulated. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Regulation of enzymes involved in methionine biosynthesis in Corynebacterium glutamicum | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.scopusid | 2-s2.0-2442466798 | - |
dc.identifier.wosid | 000221188800024 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.14, no.2, pp 373 - 378 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 14 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 373 | - |
dc.citation.endPage | 378 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001101835 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | CYSTATHIONINE GAMMA-SYNTHASE | - |
dc.subject.keywordPlus | ACETYL-L-HOMOSERINE | - |
dc.subject.keywordPlus | FERMENTATION PRODUCTION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | HOMOCYSTEINE | - |
dc.subject.keywordPlus | MUTANTS | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | FLAVUM | - |
dc.subject.keywordPlus | PLANTS | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | methionine | - |
dc.subject.keywordAuthor | homoserine acetyltransferase | - |
dc.subject.keywordAuthor | cystathionine gamma-synthase | - |
dc.subject.keywordAuthor | cystathionine beta-lyase | - |
dc.identifier.url | http://kiss.kstudy.com/thesis/thesis-view.asp?key=2219603 | - |
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