Efficient induction of T helper type 1-mediated immune responses in antigen-primed mice by anti-CD3 single-chain Fv/interleukin-18 fusion DNA
DC Field | Value | Language |
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dc.contributor.author | Kim, EJ | - |
dc.contributor.author | Cho, D | - |
dc.contributor.author | Kim, TS | - |
dc.date.available | 2021-02-22T16:03:16Z | - |
dc.date.issued | 2004-01 | - |
dc.identifier.issn | 0019-2805 | - |
dc.identifier.issn | 1365-2567 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15909 | - |
dc.description.abstract | Two types of T helper (Th) cells - Th1 and Th2 - play different roles in protection and immunopathology. The Th1 cell-mediated immune response plays an important role in inducing the host defence against intracellular bacteria and also in cancer immunotherapy. To effectively induce Th1 immune responses, we constructed a mammalian expression plasmid (pAnti-CD3sFv/IL-18) carrying a fusion gene in which anti-CD3 single-chain Fv (sFv) cDNA, the smallest unit of antibody recognizing the CD3 epsilon moiety of the T-cell receptor, was covalently linked to mature interleukin (IL)-18 cDNA. Intramuscular injection of ovalbumin (OVA)-sensitized BALB/c mice with pAnti-CD3sFv/IL-18 DNA efficiently increased the production of both OVA-specific interferon-gamma and anti-OVA immunoglobulin G2a, compared to injection with pAnti-CD3sFv DNA. In addition, pAnti-CD3sFv/IL-18 was more efficient than a mixture of pAnti-CD3sFv + pIL-18 in inducing OVA-specific, Th1 immune responses and also in inhibiting OVA-specific, IL-4 production. These studies indicate that vaccination with pAnti-CD3sFv/IL-18 fusion DNA efficiently induces the Th1 immune response in antigen-sensitized mice. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Efficient induction of T helper type 1-mediated immune responses in antigen-primed mice by anti-CD3 single-chain Fv/interleukin-18 fusion DNA | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1111/j.1365-2567.2004.01784.x | - |
dc.identifier.scopusid | 2-s2.0-0347555461 | - |
dc.identifier.wosid | 000187406100006 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY, v.111, no.1, pp 27 - 34 | - |
dc.citation.title | IMMUNOLOGY | - |
dc.citation.volume | 111 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 27 | - |
dc.citation.endPage | 34 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | INTERFERON-GAMMA PRODUCTION | - |
dc.subject.keywordPlus | DIRECT GENE-TRANSFER | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | B-CELLS | - |
dc.subject.keywordPlus | INTERLEUKIN-18 | - |
dc.subject.keywordPlus | IL-18 | - |
dc.subject.keywordPlus | TH1 | - |
dc.subject.keywordPlus | INJECTION | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | ACTIVATION | - |
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