Characterization and regulation of the gamma-glutamyl transpeptidase gene from the fission yeast Schizosaccharomyces pombe
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, HJ | - |
dc.contributor.author | Lim, HW | - |
dc.contributor.author | Kim, K | - |
dc.contributor.author | Kim, IH | - |
dc.contributor.author | Park, EH | - |
dc.contributor.author | Lim, CJ | - |
dc.date.available | 2021-02-22T16:03:16Z | - |
dc.date.issued | 2004-01 | - |
dc.identifier.issn | 0008-4166 | - |
dc.identifier.issn | 1480-3275 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15910 | - |
dc.description.abstract | The structural gene for the putative gamma-glutamyl transpeptidase (GGT) was isolated from the chromosomal DNA of the fission yeast Schizosaccharomyces pombe. The determined sequence contained 3324 bp and encoded the predicted 630 amino acid sequence of GGT, which resembles counterparts in Homo sapiens, Rattus norvegicus, Saccharomyces cerevisiae, and Escherichia coli. The S. pombe cells harboring the cloned GGT gene showed about twofold higher GGT activity in the exponential phase than the cells harboring the vector only, indicating that the cloned GGT gene was functional. To monitor the expression of the S. pombe GGT gene, we fused the fragment 1085 bp upstream of the cloned GGT gene into the promoterless beta-galactosidase gene of the shuttle vector YEp367R to generate the fusion plasmid pGT98. The synthesis of beta-galactosidase from the fusion plasmid in S. pombe cells was enhanced by treatments with NO-generating sodium nitroprusside (SN), L-buthionine-(S,R)-sulfoximine (BSO), and glycerol. The GGT mRNA level in the S. pombe cells was increased by SN and BSO. Involvement of Pap1 in the induction of the GGT gene by SN and BSO was observed. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS | - |
dc.title | Characterization and regulation of the gamma-glutamyl transpeptidase gene from the fission yeast Schizosaccharomyces pombe | - |
dc.type | Article | - |
dc.publisher.location | 캐나다 | - |
dc.identifier.doi | 10.1139/W03-106 | - |
dc.identifier.scopusid | 2-s2.0-1942506477 | - |
dc.identifier.wosid | 000189259100007 | - |
dc.identifier.bibliographicCitation | CANADIAN JOURNAL OF MICROBIOLOGY, v.50, no.1, pp 61 - 66 | - |
dc.citation.title | CANADIAN JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 50 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 61 | - |
dc.citation.endPage | 66 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | TRANSFERASE GENE | - |
dc.subject.keywordPlus | LACZ FUSIONS | - |
dc.subject.keywordPlus | GLUTATHIONE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | fission yeast | - |
dc.subject.keywordAuthor | genomic DNA | - |
dc.subject.keywordAuthor | gamma-glutamyl transpeptidase | - |
dc.subject.keywordAuthor | Pap1 | - |
dc.subject.keywordAuthor | regulation | - |
dc.subject.keywordAuthor | Schizosaccharomyces pombe | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.