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Identification and characterization of a novel (S)-ketoprofen-specific esterase
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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | 윤상영 | - |
| dc.contributor.author | 김승범 | - |
| dc.contributor.author | 유연우 | - |
| dc.contributor.author | 김두헌 | - |
| dc.date.accessioned | 2023-12-18T10:31:46Z | - |
| dc.date.available | 2023-12-18T10:31:46Z | - |
| dc.date.issued | 2007-06 | - |
| dc.identifier.issn | 0141-8130 | - |
| dc.identifier.issn | 1879-0003 | - |
| dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/159246 | - |
| dc.description.abstract | A new (S)-ketoprofen specific esterase (EST-Y29) was identified from a metagenome library from environmental samples, which showed homologies with class C-beta lactamase, penicillin binding protein, and other lipases/esterases. In order to investigate the biochemical and biophysical properties, the recombinant protein was overexpressed, purified to homogeneity, and characterized. This EST-Y29 has high catalytic activity against p-nitrophenyl esters of short fatty acids (C-2 and C-4) and alpha-naphthyl acetate with activation energy of 30.40/mol. We have further characterized EST-Y29 using high performance liquid chromatography (HPLC), circular dichroism (CD), dynamic light scattering (DLS) and size exclusion chromatography (SEC). (c) 2006 Published by Elsevier B.V. | - |
| dc.format.extent | 7 | - |
| dc.language | 영어 | - |
| dc.language.iso | ENG | - |
| dc.publisher | Elsevier BV | - |
| dc.title | Identification and characterization of a novel (S)-ketoprofen-specific esterase | - |
| dc.type | Article | - |
| dc.publisher.location | 네델란드 | - |
| dc.identifier.doi | 10.1016/j.ijbiomac.2006.11.010 | - |
| dc.identifier.scopusid | 2-s2.0-33947519464 | - |
| dc.identifier.wosid | 000246089000001 | - |
| dc.identifier.bibliographicCitation | International Journal of Biological Macromolecules, v.41, no.1, pp 1 - 7 | - |
| dc.citation.title | International Journal of Biological Macromolecules | - |
| dc.citation.volume | 41 | - |
| dc.citation.number | 1 | - |
| dc.citation.startPage | 1 | - |
| dc.citation.endPage | 7 | - |
| dc.description.isOpenAccess | N | - |
| dc.description.journalRegisteredClass | scie | - |
| dc.description.journalRegisteredClass | scopus | - |
| dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S014181300600328X?via%3Dihub | - |
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