Effect of Various Pathological Conditions on Nitric Oxide Level and L-Citrulline Uptake in Motor Neuron-Like (NSC-34) Cell Lines
DC Field | Value | Language |
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dc.contributor.author | Gautam, Shashi | - |
dc.contributor.author | Latif, Sana | - |
dc.contributor.author | Kang, Young-Sook | - |
dc.date.accessioned | 2024-04-09T02:00:26Z | - |
dc.date.available | 2024-04-09T02:00:26Z | - |
dc.date.issued | 2024-01 | - |
dc.identifier.issn | 1976-9148 | - |
dc.identifier.issn | 2005-4483 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/159846 | - |
dc.description.abstract | Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disorder that causes progressive paralysis. L-Citrulline is a non-essential neutral amino acid produced by L-arginine via nitric oxide synthase (NOS). According to previous studies, the patho-genesis of ALS entails glutamate toxicity, oxidative stress, protein misfolding, and neurofilament disruption. In addition, L-citrulline prevents neuronal cell death in brain ischemia; therefore, we investigated the change in the transport of L-citrulline under various pathological conditions in a cell line model of ALS. We examined the uptake of [14C]L-citrulline in wild-type (hSOD1wt/WT) and mutant NSC-34/ SOD1G93A (MT) cell lines. The cell viability was determined via MTT assay. A transport study was performed to determine the uptake of [14C]L-citrulline. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was performed to determine the expression levels of rat large neutral amino acid transported 1 (rLAT1) in ALS cell lines. Nitric oxide (NO) assay was performed using Griess reagent. L-Citrulline had a restorative effect on glutamate induced cell death, and increased [14C]L-citrulline uptake and mRNA levels of the large neutral amino acid transporter (LAT1) in the glutamate-treated ALS disease model (MT). NO levels increased significantly when MT cells were pretreated with glutamate for 24 h and restored by co-treatment with L-citrulline. Co-treatment of MT cells with L-arginine, an NO donor, increased NO levels. NSC-34 cells exposed to high glucose conditions showed a significant increase in [14 C]L-citrulline uptake and LAT1 mRNA expression levels, which were restored to normal levels upon co-treatment with unlabeled L-citrulline. In contrast, exposure of the MT cell line to tumor necrosis factor al-pha, lipopolysaccharides, and hypertonic condition decreased the uptake significantly which was restored to the normal level by co-treating with unlabeled L-citrulline. L-Citrulline can restore NO levels and cellular uptake in ALS-affected cells with glutamate cytotoxicity, pro-inflammatory cytokines, or other pathological states, suggesting that L-citrulline supplementation in ALS may play a key role in providing neuroprotection. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Korean Society of Applied Pharmacology | - |
dc.title | Effect of Various Pathological Conditions on Nitric Oxide Level and L-Citrulline Uptake in Motor Neuron-Like (NSC-34) Cell Lines | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.4062/biomolther.2023.110 | - |
dc.identifier.scopusid | 2-s2.0-85180904083 | - |
dc.identifier.wosid | 001177628900002 | - |
dc.identifier.bibliographicCitation | Biomolecules and Therapeutics, v.32, no.1, pp 154 - 161 | - |
dc.citation.title | Biomolecules and Therapeutics | - |
dc.citation.volume | 32 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 154 | - |
dc.citation.endPage | 161 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART003027998 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | BLOOD-BRAIN-BARRIER | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | TAURINE UPTAKE | - |
dc.subject.keywordPlus | SYNTHASE | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | ARGININE | - |
dc.subject.keywordPlus | HEALTH | - |
dc.subject.keywordPlus | SERINE | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordAuthor | Amyotrophic lateral sclerosis | - |
dc.subject.keywordAuthor | Glutamate | - |
dc.subject.keywordAuthor | Hypoxia-inducible factor | - |
dc.subject.keywordAuthor | L-citrulline | - |
dc.subject.keywordAuthor | Large neutral amino acid transporter 1 | - |
dc.subject.keywordAuthor | Nitric oxide | - |
dc.identifier.url | https://www.biomolther.org/journal/view.html?doi=10.4062/biomolther.2023.110 | - |
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