L-Ascorbic acid (vitamin C) induces the apoptosis of B16 murine melanoma cells via a caspase-8-independent pathway
DC Field | Value | Language |
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dc.contributor.author | Kang, JS | - |
dc.contributor.author | Cho, DH | - |
dc.contributor.author | Kim, YI | - |
dc.contributor.author | Hahm, E | - |
dc.contributor.author | Yang, YH | - |
dc.contributor.author | Kim, D | - |
dc.contributor.author | Hur, D | - |
dc.contributor.author | Park, H | - |
dc.contributor.author | Bang, S | - |
dc.contributor.author | Hwang, YI | - |
dc.contributor.author | Lee, WJ | - |
dc.date.available | 2021-02-22T16:17:06Z | - |
dc.date.issued | 2003-11 | - |
dc.identifier.issn | 0340-7004 | - |
dc.identifier.issn | 1432-0851 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16153 | - |
dc.description.abstract | L-Ascorbic acid (vitamin C) has been reported to play a role in the treatment and prevention of cancer. However, its specific mechanistic pathways remain obscure. This study was carried out to identify the sodium ascorbate-induced apoptotic pathway in B16F10 murine melanoma cells. Sodium ascorbate was found to induce the apoptosis of B16F10 murine melanoma in a time- and dose-dependent manner, and this was prevented by pretreatment with N-acetyl-L-cysteine (NAC), a well-known antioxidant. In fact, sodium ascorbate-treated B16F10 melanoma cells showed increased intracellular reactive oxygen species generation (ROS) levels. These results indicate that sodium ascorbate induced apoptosis in B16F10 murine melanoma cells by acting as a prooxidant. We examined the involvement of caspase-8 using a specific caspase-8 inhibitor (z-IETD-fmk) on the sodium ascorbate-induced apoptotic pathway. Cell death was found not to be inhibited by z-IETD-fmk treatment, indicating that sodium ascorbate-induced apoptosis is not mediated by caspase-8. In addition, we detected a reduction in the mitochondrial membrane potential during apoptosis and confirmed cytochrome-c release from mitochondria by immunoblotting. Taken together, it appears that the induction of a prooxidant state by sodium ascorbate and a subsequent reduction in mitochondrial membrane potential are involved in the apoptotic pathway of B16F10 murine melanoma cells, and that this occurs in a caspase-8-independent manner. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER | - |
dc.title | L-Ascorbic acid (vitamin C) induces the apoptosis of B16 murine melanoma cells via a caspase-8-independent pathway | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1007/s00262-003-0407-6 | - |
dc.identifier.scopusid | 2-s2.0-0242329380 | - |
dc.identifier.wosid | 000185976200006 | - |
dc.identifier.bibliographicCitation | CANCER IMMUNOLOGY IMMUNOTHERAPY, v.52, no.11, pp 693 - 698 | - |
dc.citation.title | CANCER IMMUNOLOGY IMMUNOTHERAPY | - |
dc.citation.volume | 52 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 693 | - |
dc.citation.endPage | 698 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | CYTOCHROME-C | - |
dc.subject.keywordPlus | SERINE-PROTEASE | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | CASPASES | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | INTERFERON | - |
dc.subject.keywordAuthor | vitamin C | - |
dc.subject.keywordAuthor | melanoma | - |
dc.subject.keywordAuthor | apoptosis | - |
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