Conjugated linoleic acid inhibits cell proliferation and ErbB3 signaling in HT-29 human colon cell line
DC Field | Value | Language |
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dc.contributor.author | Cho, HJ | - |
dc.contributor.author | Kim, WK | - |
dc.contributor.author | Kim, EJ | - |
dc.contributor.author | Jung, KC | - |
dc.contributor.author | Park, S | - |
dc.contributor.author | Lee, HS | - |
dc.contributor.author | Tyner, AL | - |
dc.contributor.author | Park, JHY | - |
dc.date.available | 2021-02-22T16:17:33Z | - |
dc.date.issued | 2003-06 | - |
dc.identifier.issn | 0193-1857 | - |
dc.identifier.issn | 1522-1547 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16186 | - |
dc.description.abstract | Conjugated linoleic acid (CLA) has chemoprotective properties in experimental cancer models, and in vitro studies have shown that CLA inhibits HT-29 colon cancer cell growth. ErbB2 and ErbB3 have been implicated in the development of colon cancer, and both proteins are expressed at high levels in the HT-29 cell line. Activation of ErbB2/ErbB3 heterodimers is regulated by the ErbB3 ligand heregulin. To examine CLA regulation of HT-29 cell proliferation and apoptosis and the influence of CLA on the ErbB3 signaling pathway, HT-29 cells were cultured in the presence of CLA and/or heregulin. CLA inhibited DNA synthesis and induced apoptosis of HT-29 cells. Although the addition of heregulin-alpha led to an increase in cell number, it was not able to counteract the negative growth regulatory effect of CLA. Immunoprecipitation/Western blot studies revealed that CLA inhibited heregulin-alpha-stimulated phosphorylation of ErbB2 and ErbB3, recruitment of the p85 subunit of phosphoinositide 3-kinase (PI3-kinase) to the ErbB3 receptor, ErbB3-associated PI3-kinase activities, and phosphorylation of Akt. CLA decreased ErbB2 and ErbB3 mRNA and protein levels in a dose-dependent manner. In conclusion, we demonstrate that CLA inhibits cell proliferation and stimulates apoptosis in HT-29 cells and that this may be mediated by its ability to downregulate ErbB3 signaling and the PI3-kinase/ Akt pathway. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER PHYSIOLOGICAL SOC | - |
dc.title | Conjugated linoleic acid inhibits cell proliferation and ErbB3 signaling in HT-29 human colon cell line | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1152/ajpgi.00347.2002 | - |
dc.identifier.scopusid | 2-s2.0-0038586230 | - |
dc.identifier.wosid | 000182699700017 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, v.284, no.6, pp G996 - G1005 | - |
dc.citation.title | AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | - |
dc.citation.volume | 284 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | G996 | - |
dc.citation.endPage | G1005 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.subject.keywordPlus | ACTIVATED RECEPTOR-GAMMA | - |
dc.subject.keywordPlus | TYROSINE KINASE-ACTIVITY | - |
dc.subject.keywordPlus | EPIDERMAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | PLECKSTRIN HOMOLOGY DOMAIN | - |
dc.subject.keywordPlus | HUMAN PROSTATE-CANCER | - |
dc.subject.keywordPlus | PHOSPHATIDYLINOSITOL 3-KINASE | - |
dc.subject.keywordPlus | COLORECTAL-CANCER | - |
dc.subject.keywordPlus | BLOCKS APOPTOSIS | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordAuthor | heregulin | - |
dc.subject.keywordAuthor | phosphoinositide 3-kinase | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.identifier.url | https://journals.physiology.org/doi/full/10.1152/ajpgi.00347.2002 | - |
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