Pap1-dependent regulation of the GSTII gene from the fission yeast
DC Field | Value | Language |
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dc.contributor.author | Lim, CJ | - |
dc.contributor.author | Cho, YW | - |
dc.contributor.author | Sa, JH | - |
dc.contributor.author | Lim, HW | - |
dc.contributor.author | Kim, HG | - |
dc.contributor.author | Kim, SJ | - |
dc.contributor.author | Park, EH | - |
dc.date.available | 2021-02-22T16:31:12Z | - |
dc.date.issued | 2002-12 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16369 | - |
dc.description.abstract | The genomic DNA encoding a second glutathione S-transferase (GSTII) was previously isolated from the fission yeast Schizosaccharomyces pombe. Its expression was shown to be induced by menadione, mercuric chloride, o-dinitrobenzene, and NO-generating S-nitroso-N-acetylpenicillamine using the GSTII-lacZ fusion harboring the 910 bp upstream region from the translational initiation point. In this study, the additional fusion plasmids pGST50-590 and pGST50-6R590 were constructed to carry the 590 bp upstream region in the vectors YEp357 and YEp367R, respectively. The synthesis of P-galactosidase from the fusion plasmid pGST50-590 was about 3-fold higher than that from the fusion plasmid pGST50-F, indicating the presence of negatively activating sequence in the -910 similar to -590 region. It was also enhanced by the same agents, which induced the synthesis of beta-galactosidase from the fusion plasmid pGST50-F. The synthesis of beta-galactosidase from both fusion plasmids pGST50-F and pGST50-590 was enhanced by the overexpressed Pap1 protein. The synthesis of P-galactosidase from the two YEp367R derivatives pGST50-6R-F and pGST50-6R-590 was greatly decreased in the Pap1-negative strain TP108-3C. These results propose the Pap1-dependent regulation of the GSTII gene from the fission yeast. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER-VERLAG SINGAPORE PTE LTD | - |
dc.title | Pap1-dependent regulation of the GSTII gene from the fission yeast | - |
dc.type | Article | - |
dc.publisher.location | 싱가폴 | - |
dc.identifier.scopusid | 2-s2.0-0038652143 | - |
dc.identifier.wosid | 000180215300016 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.14, no.3, pp 431 - 436 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 14 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 431 | - |
dc.citation.endPage | 436 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001189258 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | GLUTATHIONE-S-TRANSFERASE | - |
dc.subject.keywordPlus | SCHIZOSACCHAROMYCES-POMBE | - |
dc.subject.keywordPlus | THIOLTRANSFERASE | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | SUBSTRATE | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordAuthor | fission yeast | - |
dc.subject.keywordAuthor | glutathione S-transferase | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | Pap1 | - |
dc.subject.keywordAuthor | regulation | - |
dc.subject.keywordAuthor | Schizosaccharomyces pombe | - |
dc.identifier.url | https://web.p.ebscohost.com/ehost/detail/detail?vid=8&sid=a755d46f-5f6e-43ee-9c53-68b324faf84a%40redis&bdata=Jmxhbmc9a28mc2l0ZT1laG9zdC1saXZlJnNjb3BlPXNpdGU%3d#AN=9742956&db=a9h | - |
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