Regulation of peroxiredoxin I activity by Cdc2-mediated phosphorylation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chang T.-S. | - |
dc.contributor.author | Jeong W. | - |
dc.contributor.author | Choi S.Y. | - |
dc.contributor.author | Yu S. | - |
dc.contributor.author | Kang S.W. | - |
dc.contributor.author | Rhee S.G. | - |
dc.date.available | 2021-02-22T16:33:50Z | - |
dc.date.issued | 2002-07 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16606 | - |
dc.description.abstract | Hydrogen peroxide is implicated as an intracellular messenger in various cellular responses such as proliferation and differentiation. Peroxiredoxin (Prx) I is a member of the peroxiredoxin family of peroxidases and contains a consensus site (Thr90-Pro-Lys-Lys) for phosphorylation by cyclin-dependent kinases (CDKs). This protein has now been shown to be phosphorylated specifically on Thr90 by several CDKs, including Cdc2, in vitro. Phosphorylation of Prx I on Thr90 reduced the peroxidase activity of this protein by 80%. The phosphorylation of Prx I in HeLa cells was monitored with the use of antibodies specific for Prx I phosphorylated on Thr90. Immunoblot analysis with these antibodies of HeLa cells arrested at various stages of the cell cycle revealed that Prx I phosphorylation occurs in parallel with the activation of Cdc2; Prx I phosphorylation was thus marked during mitosis but virtually undetectable during interphase. Furthermore, when Cdc2 expression was reduced by RNA interference with cognate small interfering RNAs, Prx I phosphorylation was not observed in the cells synchronized in mitotic phase. The cytosolic location of Prx I likely prevents its interaction with activated CDKs until after the breakdown of the nuclear envelope during mitosis, when Cdc2 is the CDK that is most active. Phosphorylation of Prx I on Thr90 both in vitro and in vivo was blocked by roscovitine, an inhibitor of CDKs. These results suggest that Cdc2-mediated phosphorylation and inactivation of Prx I and the resulting intracellular accumulation of H2O2 might be important for progression of the cell cycle. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Regulation of peroxiredoxin I activity by Cdc2-mediated phosphorylation | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1074/jbc.M110432200 | - |
dc.identifier.scopusid | 2-s2.0-0037067763 | - |
dc.identifier.wosid | 000176747000069 | - |
dc.identifier.bibliographicCitation | Journal of Biological Chemistry, v.277, no.28, pp 25370 - 25376 | - |
dc.citation.title | Journal of Biological Chemistry | - |
dc.citation.volume | 277 | - |
dc.citation.number | 28 | - |
dc.citation.startPage | 25370 | - |
dc.citation.endPage | 25376 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | Antibodies | - |
dc.subject.keywordPlus | Cell culture | - |
dc.subject.keywordPlus | Enzymes | - |
dc.subject.keywordPlus | Hydrogen peroxide | - |
dc.subject.keywordPlus | Immunology | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | Cell cycles | - |
dc.subject.keywordPlus | Phosphorylations | - |
dc.subject.keywordPlus | Biochemistry | - |
dc.subject.keywordPlus | cyclin dependent kinase 2 | - |
dc.subject.keywordPlus | hydrogen peroxide | - |
dc.subject.keywordPlus | peroxiredoxin | - |
dc.subject.keywordPlus | roscovitine | - |
dc.subject.keywordPlus | antibody specificity | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell growth | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | enzyme activity | - |
dc.subject.keywordPlus | enzyme inactivation | - |
dc.subject.keywordPlus | enzyme phosphorylation | - |
dc.subject.keywordPlus | flow cytometry | - |
dc.subject.keywordPlus | HeLa cell | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | immunoblotting | - |
dc.subject.keywordPlus | interphase | - |
dc.subject.keywordPlus | mitosis | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | regulatory mechanism | - |
dc.subject.keywordPlus | Amino Acid Sequence | - |
dc.subject.keywordPlus | CDC2 Protein Kinase | - |
dc.subject.keywordPlus | Cell Cycle | - |
dc.subject.keywordPlus | Hela Cells | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Peroxidases | - |
dc.subject.keywordPlus | Phosphorylation | - |
dc.subject.keywordPlus | Threonine | - |
dc.identifier.url | https://www.jbc.org/article/S0021-9258(19)66496-3/fulltext | - |
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