Kazinol U inhibits melanogenesis through the inhibition of tyrosinase-related proteins via AMP kinase activation
DC Field | Value | Language |
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dc.contributor.author | Lim, Jihyun | - |
dc.contributor.author | Nam, Sorim | - |
dc.contributor.author | Jeong, Ji Hye | - |
dc.contributor.author | Kim, Min Jung | - |
dc.contributor.author | Yang, Young | - |
dc.contributor.author | Lee, Myeong-Sok | - |
dc.contributor.author | Lee, Hee Gu | - |
dc.contributor.author | Ryu, Jae-Ha | - |
dc.contributor.author | Lim, Jong-Seok | - |
dc.date.available | 2021-02-22T06:45:47Z | - |
dc.date.issued | 2019-03 | - |
dc.identifier.issn | 0007-1188 | - |
dc.identifier.issn | 1476-5381 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/3745 | - |
dc.description.abstract | Background and Purpose Kazinol U is a prenylated flavan isolated from an extract of Broussonetia kazinoki Sieb (Moraceae). Kazinol U has shown cytoprotective effects against cytokine-induced apoptotic cell death and induces AMP kinase (AMPK) activation through LKB1 activation. However, kazinol U has not been tested as a regulator of melanogenesis, although bark extract of B. kazinoki has been used as a cosmetic ingredient for skin conditioning. Experimental Approach We cultured mouse, human melanoma cells and normal human melanocytes to demonstrate anti-melanogenic effects of kazinol U. A tyrosinase activity assay, Western blot, RT-qPCR and a luciferase reporter gene assay were performed to determine the anti-melanogenic mechanisms of kazinol U. We confirmed its effect on melanogenesis in vivo using zebrafish. Key Results Kazinol U inhibited the expression and activity of tyrosinase, the rate-limiting enzyme in melanogenesis, and reduced tyrosinase expression and activity in response to cAMP-inducing agents. Kazinol U reduced the expression of other melanogenic enzymes, such as tyrosinase-related protein (Tyrp) 1 and Tyrp2, and down-regulated microphthalmia-associated transcription factor (MITF), the master regulator of the tyrosinase gene family. Moreover, kazinol U induced phosphorylation of AMPK and MAPK proteins, which are MITF inhibitors. It also exhibited anti-melanogenic effects in zebrafish, a recently developed in vivo model. CONCLUSIONS AND IMPLICATIONS Our findings suggest that kazinol U reduces melanogenesis via its inhibitory effect on MITF and its downstream target genes, tyrosinase, Tyrp1 and Tyrp2. This work may provide a basis for the application of kazinol U for the treatment of hyperpigmentation skin disorders. | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Kazinol U inhibits melanogenesis through the inhibition of tyrosinase-related proteins via AMP kinase activation | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1111/bph.14560 | - |
dc.identifier.scopusid | 2-s2.0-85060586299 | - |
dc.identifier.wosid | 000458528300005 | - |
dc.identifier.bibliographicCitation | BRITISH JOURNAL OF PHARMACOLOGY, v.176, no.5, pp 737 - 750 | - |
dc.citation.title | BRITISH JOURNAL OF PHARMACOLOGY | - |
dc.citation.volume | 176 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 737 | - |
dc.citation.endPage | 750 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | MELANIN BIOSYNTHESIS | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | CONCISE GUIDE | - |
dc.subject.keywordPlus | ZEBRAFISH | - |
dc.subject.keywordPlus | MITF | - |
dc.subject.keywordPlus | SKIN | - |
dc.subject.keywordPlus | PIGMENTATION | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.identifier.url | https://bpspubs.onlinelibrary.wiley.com/doi/full/10.1111/bph.14560 | - |
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