Single-molecule DNA visualization using AT-specific red and non-specific green DNA-binding fluorescent proteins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jihyun | - |
dc.contributor.author | Lee, Seonghyun | - |
dc.contributor.author | Won, Nabin | - |
dc.contributor.author | Shin, Eunji | - |
dc.contributor.author | Kim, Soo-Hyun | - |
dc.contributor.author | Chun, Min-Young | - |
dc.contributor.author | Gu, Jungyeun | - |
dc.contributor.author | Jung, Gun-Young | - |
dc.contributor.author | Lim, Kwang-Il | - |
dc.contributor.author | Jo, Kyubong | - |
dc.date.available | 2021-02-22T06:46:06Z | - |
dc.date.issued | 2019-02 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.issn | 1364-5528 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/3835 | - |
dc.description.abstract | The recent advances in the single cell genome analysis are generating a considerable amount of novel insights into complex biological systems. However, there are still technical challenges because each cell has a single copy of DNA to be amplified in most single cell genome analytical methods. In this paper, we present a novel approach to directly visualize a genomic map on a large DNA molecule instantly stained with red and green DNA-binding fluorescent proteins without DNA amplification. For this visualization, we constructed a few types of fluorescent protein-fused DNA-binding proteins: H-NS (histone-like nucleoid-structuring protein), DNA-binding domain of BRCA1 (breast cancer 1), high mobility group-1 (HMG), and lysine tryptophan (KW) repeat motif. Because H-NS and HMG preferentially bind A/T-rich regions, we combined A/T specific binder (H-NS-mCherry and HMG-mCherry as red color) and a non-specific complementary DNA binder (BRCA1-eGFP and 2(KW)(2)-eGFP repeat as green color) to produce a sequence-specific two-color DNA physical map for efficient optical identification of single DNA molecules. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Single-molecule DNA visualization using AT-specific red and non-specific green DNA-binding fluorescent proteins | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1039/c8an01426d | - |
dc.identifier.scopusid | 2-s2.0-85060618103 | - |
dc.identifier.wosid | 000457394400013 | - |
dc.identifier.bibliographicCitation | ANALYST, v.144, no.3, pp 921 - 927 | - |
dc.citation.title | ANALYST | - |
dc.citation.volume | 144 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 921 | - |
dc.citation.endPage | 927 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | GENOME | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2019/AN/C8AN01426D#!divAbstract | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.