Caspase-4 disaggregates lipopolysaccharide micelles via LPS-CARD interaction
DC Field | Value | Language |
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dc.contributor.author | An, Jinsu | - |
dc.contributor.author | Kim, Seong Ho | - |
dc.contributor.author | Hwang, Dohyeon | - |
dc.contributor.author | Lee, Kyung Eun | - |
dc.contributor.author | Kim, Min Jung | - |
dc.contributor.author | Yang, Eun Gyeong | - |
dc.contributor.author | Kim, So Yeon | - |
dc.contributor.author | Chung, Hak Suk | - |
dc.date.available | 2021-02-22T06:46:17Z | - |
dc.date.issued | 2019-01 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/3881 | - |
dc.description.abstract | Lipopolysaccharides (LPS) are a major component of the outer membrane of Gram-negative bacteria and are pathogen-associated molecular patterns recognized by the TLR4/MD2 complex that induces an inflammatory response. Recently, the cytosolic receptors caspase-4/-5/-11 that bind LPS inside the cell and trigger inflammasome activation or pyroptosis, have been identified. Despite the important roles of caspase-4 in human immune responses, few studies have investigated its biochemical characteristics and interactions with LPS. Since caspase-4 (C258A) purified from an Escherichia coli host forms aggregates, monomeric proteins including full-length caspase-4, caspase-4 (C258A), and the CARD domain of caspase-4 have been purified from the insect cell system. Here, we report the overexpression and purification of monomeric caspase-4 (C258A) and CARD domain from E. coli and demonstrate that purified caspase-4 (C258A) and CARD domain bind large LPS micelles and disaggregate them to small complexes. As the molar ratio of caspase-4 to LPS increases, the size of the caspase-4/LPS complex decreases. Our results present a new function of caspase-4 and set the stage for structural and biochemical studies, and drug discovery targeting LPS/caspase-4 interactions by establishing a facile purification method to obtain large quantities of purified caspase-4 (C258A) and the CARD domain. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Caspase-4 disaggregates lipopolysaccharide micelles via LPS-CARD interaction | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1038/s41598-018-36811-4 | - |
dc.identifier.scopusid | 2-s2.0-85060828947 | - |
dc.identifier.wosid | 000456955500006 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.9 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 9 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.identifier.url | https://www.nature.com/articles/s41598-018-36811-4 | - |
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