Nanowire Aptasensors for Electrochemical Detection of Cell-Secreted Cytokines
DC Field | Value | Language |
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dc.contributor.author | Liu, Ying | - |
dc.contributor.author | Rahimian, Ali | - |
dc.contributor.author | Krylyuk, Sergiy | - |
dc.contributor.author | Vu, Tam | - |
dc.contributor.author | Crulhas, Bruno | - |
dc.contributor.author | Stybayeva, Gulnaz | - |
dc.contributor.author | Imanbekova, Meruyert | - |
dc.contributor.author | Shin, Dong-Sik | - |
dc.contributor.author | Davydov, Albert | - |
dc.contributor.author | Revzin, Alexander | - |
dc.date.available | 2021-02-22T10:46:25Z | - |
dc.date.issued | 2017-11 | - |
dc.identifier.issn | 2379-3694 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/5052 | - |
dc.description.abstract | Cytokines are small proteins secreted by immune cells in response to pathogens/infections; therefore, these proteins can be used in diagnosing infectious diseases. For example, release of a cytokine interferon (IFN)-gamma from T-cells is used for blood-based diagnosis of tuberculosis (TB). Our lab has previously developed an atpamer-based electrochemical biosensor for rapid and sensitive detection of IFN-gamma. In this study, we explored the use of silicon nanowires (NWs) as a way to create nanostructured electrodes with enhanced sensitivity for IFN-gamma. Si NWs were covered with gold and were further functionalized with thiolated aptamers specific for IFN-gamma. Aptamer molecules were designed to form a hairpin and in addition to terminal thiol groups contained redox reporter molecules methylene blue. Binding of analyte to aptamer-modified NWs (termed here nanowire aptasensors) inhibited electron transfer from redox reporters to the electrode and caused electrochemical redox signal to decrease. In a series of experiments we demonstrate that NW aptasensors responded 3x faster and were 2x more sensitive to IFN-gamma compared to standard flat electrodes. Most significantly, NW aptasensors allowed detection of IFN-gamma from as few as 150 T-cells/mL while ELISA did not pick up signal from the same number of cells. One of the challenges faced by ELISA-based TB diagnostics is poor performance in patients whose T-cell numbers are low, typically HIV patients. Therefore, NW aptasensors developed here may be used in the future for more sensitive monitoring of IFN-gamma responses in patients coinfected with HIV/TB. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Nanowire Aptasensors for Electrochemical Detection of Cell-Secreted Cytokines | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/acssensors.7b00486 | - |
dc.identifier.scopusid | 2-s2.0-85034840106 | - |
dc.identifier.wosid | 000416499400015 | - |
dc.identifier.bibliographicCitation | ACS SENSORS, v.2, no.11, pp 1644 - 1652 | - |
dc.citation.title | ACS SENSORS | - |
dc.citation.volume | 2 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1644 | - |
dc.citation.endPage | 1652 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | RECONFIGURABLE MICROFLUIDICS | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | BIOMOLECULES | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | SENSORS | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordAuthor | electrochemical biosensor | - |
dc.subject.keywordAuthor | IFN-gamma aptamer | - |
dc.subject.keywordAuthor | nanowire electrode | - |
dc.subject.keywordAuthor | aptasensor | - |
dc.subject.keywordAuthor | tuberculosis detection | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acssensors.7b00486 | - |
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