An electrochemical aptasensor for detection of bovine interferon gamma
DC Field | Value | Language |
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dc.contributor.author | Crulhas, Bruno P. | - |
dc.contributor.author | Hadley, Dustin | - |
dc.contributor.author | Liu, Ying | - |
dc.contributor.author | Shin, Dong-Sik | - |
dc.contributor.author | Stybayeva, Gulnaz | - |
dc.contributor.author | Imanbekova, Meruyert | - |
dc.contributor.author | Hill, Ashley E. | - |
dc.contributor.author | Pedrosa, Valber | - |
dc.contributor.author | Revzin, Alexander | - |
dc.date.available | 2021-02-22T11:12:42Z | - |
dc.date.issued | 2017-08 | - |
dc.identifier.issn | 1759-9660 | - |
dc.identifier.issn | 1759-9679 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/8228 | - |
dc.description.abstract | Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-gamma) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-gamma. A thiolated IFN-gbinding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by selfassembly. Binding of IFN-g to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-g with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | An electrochemical aptasensor for detection of bovine interferon gamma | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1039/c7ay01313b | - |
dc.identifier.scopusid | 2-s2.0-85027335841 | - |
dc.identifier.wosid | 000411506500006 | - |
dc.identifier.bibliographicCitation | ANALYTICAL METHODS, v.9, no.31, pp 4527 - 4532 | - |
dc.citation.title | ANALYTICAL METHODS | - |
dc.citation.volume | 9 | - |
dc.citation.number | 31 | - |
dc.citation.startPage | 4527 | - |
dc.citation.endPage | 4532 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.subject.keywordPlus | MYCOBACTERIUM-BOVIS | - |
dc.subject.keywordPlus | BLOOD-SERUM | - |
dc.subject.keywordPlus | TUBERCULOSIS | - |
dc.subject.keywordPlus | CATTLE | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.subject.keywordPlus | ASSAY | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2017/AY/C7AY01313B#!divAbstract | - |
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