Imperatorin is Transported through Blood-Brain Barrier by Carrier-Mediated Transporters
DC Field | Value | Language |
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dc.contributor.author | Tun, Temdara | - |
dc.contributor.author | Kang, Young-Sook | - |
dc.date.available | 2021-02-22T11:12:49Z | - |
dc.date.issued | 2017-07 | - |
dc.identifier.issn | 1976-9148 | - |
dc.identifier.issn | 2005-4483 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/8244 | - |
dc.description.abstract | Imperatorin, a major bioactive furanocoumarin with multifunctions, can be used for treating neurodegenerative diseases. In this study, we investigated the characteristics of imperatorin transport in the brain. Experiments of the present study were designed to study imperatorin transport across the blood-brain barrier both in vivo and in vitro. In vivo study was performed in rats using single intravenous injection and in situ carotid artery perfusion technique. Conditionally immortalized rat brain capillary endothelial cells were as an in vitro model of blood-brain barrier to examine the transport mechanism of imperatorin. Brain distribution volume of imperatorin was about 6 fold greater than that of sucrose, suggesting that the transport of imperatorin was through the blood-brain barrier in physiological state. Both in vivo and in vitro imperatorin transport studies demonstrated that imperatorin could be transported in a concentration-dependent manner with high affinity. Imperatorin uptake was dependent on proton gradient in an opposite direction. It was significantly reduced by pretreatment with sodium azide. However, its uptake was not inhibited by replacing extracellular sodium with potassium or N-methylglucamine. The uptake of imperatorin was inhibited by various cationic compounds, but not inhibited by TEA, choline and organic anion substances. Transfection of plasma membrane monoamine transporter, organic cation transporter 2 and organic cation/carnitine transporter 2/1. siRNA failed to alter imperatorin transport in brain capillary endothelial cells. Especially, tramadol, clonidine and pyrilamine inhibited the uptake of [H-3]imperatorin competitively. Therefore, imperatorin is actively transported from blood to brain across the blood-brain barrier by passive and carrier-mediated transporter. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC APPLIED PHARMACOLOGY | - |
dc.title | Imperatorin is Transported through Blood-Brain Barrier by Carrier-Mediated Transporters | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.4062/biomolther.2017.082 | - |
dc.identifier.scopusid | 2-s2.0-85023599109 | - |
dc.identifier.wosid | 000404947800014 | - |
dc.identifier.bibliographicCitation | BIOMOLECULES & THERAPEUTICS, v.25, no.4, pp 441 - 451 | - |
dc.citation.title | BIOMOLECULES & THERAPEUTICS | - |
dc.citation.volume | 25 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 441 | - |
dc.citation.endPage | 451 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002233177 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | ORGANIC CATION TRANSPORTER | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | FUNCTIONAL-CHARACTERIZATION | - |
dc.subject.keywordPlus | EFFLUX TRANSPORT | - |
dc.subject.keywordPlus | CELL-LINES | - |
dc.subject.keywordPlus | TAURINE TRANSPORT | - |
dc.subject.keywordPlus | INFLUX TRANSPORT | - |
dc.subject.keywordPlus | ANTIPORTER | - |
dc.subject.keywordPlus | INVOLVEMENT | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordAuthor | Imperatorin | - |
dc.subject.keywordAuthor | Alzheimer's disease | - |
dc.subject.keywordAuthor | Blood-brain barrier | - |
dc.subject.keywordAuthor | Proton coupled antiporter | - |
dc.identifier.url | http://www.biomolther.org/journal/view.html?volume=25&number=4&spage=441&year=2017 | - |
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