Lipo-PGE1 suppresses collagen production in human dermal fibroblasts via the ERK/Ets-1 signaling pathway
DC Field | Value | Language |
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dc.contributor.author | Yang, Yoolhee | - |
dc.contributor.author | Kim, Hee Jung | - |
dc.contributor.author | Woo, Kyong-Je | - |
dc.contributor.author | Cho, Daeho | - |
dc.contributor.author | Bang, Sa Ik | - |
dc.date.available | 2021-02-22T11:13:14Z | - |
dc.date.issued | 2017-06 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/8297 | - |
dc.description.abstract | Dysregulation of collagen production contributes to various pathological processes, including tissue fibrosis as well as impaired wound healing. Lipo-prostaglandin E1 (Lipo-PGE1), a lipid microsphere-incorporated prostaglandin E1, is used as a vasodilator for the treatment of peripheral vascular diseases. Lipo-PGE1 was recently shown to enhance human dermal fibroblast (HDF) migration and in vivo wound healing. No published study has characterized the role of Lipo-PGE1 in collagen regulation in HDFs. Here, we investigated the cellular signaling mechanism by which Lipo-PGE1 regulates collagen in HDFs. Collagen production was evaluated by the Sircol collagen assay, Western blot analysis of type I collagen and real time PCR. Unexpectedly, Lipo-PGE1 decreased mRNA expression of collagen 1A1, 1A2, and 3A1. Lipo-PGE1 markedly inhibited type I collagen and total soluble collagen production. In addition, Lipo-PGE1 inhibited transforming growth factor-beta-induced collagen expression via Smad2 phosphorylation. To further investigate whether extracellular signalregulated kinase (ERK)/Ets-1 signaling, a crucial pathway in collagen regulation, is involved in Lipo-PGE1-inhibited collagen production, cells were pretreated with an ERK-specific inhibitor, PD98059, prior to the addition of Lipo-PGE1. Lipo-PGE1-inhibited collagen mRNA expression and total soluble collagen production were recovered by pretreatment with PD98059. Moreover, Lipo-PGE1 directly induced the phosphorylation of ERK. Furthermore, silencing of Ets-1 recovered Lipo- PGE1-inhibited collagen production and PD98059 blocked Lipo-PGE1-enhanced Ets-1 expression. The present study reveals an important role for Lipo-PGE1 as a negative regulator of collagen gene expression and production via ERK/Ets-1 signaling. These results suggest that Lipo-PGE1 could potentially be a therapeutic target in diseases with deregulated collagen turnover. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Lipo-PGE1 suppresses collagen production in human dermal fibroblasts via the ERK/Ets-1 signaling pathway | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1371/journal.pone.0179614 | - |
dc.identifier.scopusid | 2-s2.0-85021285990 | - |
dc.identifier.wosid | 000404145100025 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.12, no.6 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 12 | - |
dc.citation.number | 6 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | CATHELICIDIN LL-37 | - |
dc.subject.keywordPlus | PEPTIDE LL-37 | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | FIBROSIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | ERK | - |
dc.subject.keywordPlus | SCLERODERMA | - |
dc.subject.keywordPlus | KELOIDS | - |
dc.identifier.url | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0179614 | - |
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