The alteration of serine transporter activity in a cell line model of amyotrophic lateral sclerosis (ALS)
DC Field | Value | Language |
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dc.contributor.author | Lee, Na-Young | - |
dc.contributor.author | Kim, Yunha | - |
dc.contributor.author | Ryu, Hoon | - |
dc.contributor.author | Kang, Young-Sook | - |
dc.date.available | 2021-02-22T11:18:07Z | - |
dc.date.issued | 2017-01 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/8929 | - |
dc.description.abstract | The alteration of D-serine levels is associated with the pathogenesis of sporadic ALS and mutant SOD1 (G93A) animal model of ALS. However, the exact mechanism of D-serine transport is not known in ALS. To better understand the distribution of D-serine in ALS, we determined the activity and the expression of serine transporter in a motor neuronal cell line model of ALS (NSC-34/hSOD1(G93A) cells). The uptake of [3H] D-serine was significantly lower in NSC-34/hSOD1(G93A) cells than in control NSC-34 and NSC-34/ hSOD1(wt) cells. In contrast, the uptake of [3H] L-serine, precursor of D-serine, was markedly increased in NSC-34/hSOD1(G93A) cells compared to control NSC-34 and NSC-34/hSOD1(wt) cells. Both [3H] D-serine and [3H] L-serine uptake were saturable in these cells. The estimated Michaelis-Menten constant, Km, for Dserine uptakes was higher in NSC-34/hSOD1(G93A) cells than in NSC-34/hSOD1(wt) cells while the Km for Lserine uptake was 2 fold lower in NSC-34/hSOD1(G93A) cells than in control cells. [3H] D-serine and [3H] Lserine uptakes took place in a Na+-dependent manner, and both uptakes were significantly inhibited by system ASC (alanine-serine-cysteine) substrates. As a result of small interfering RNA experiments, we found that ASCT2 (SLC1A5) and ASCT1 (SLC1A4) are involved in [3H] D-serine and [3H] L-serine uptake in NSC-34/hSOD1(G93A) cells, respectively. The level of SLC1A4 mRNA was significantly increased in NSC-34/ hSOD1(G93A) compared to NSC-34 and NSC-34/hSOD1(wt) cells. In contrast, the level of SLC7A10 mRNA was relatively lower in NSC-34/hSOD1(G93A) cells than the control cells. Together, these data suggest that the pathological alteration of D-and L-serine uptakes in ALS is driven by the affinity change of D-and L-serine uptake system. (C) 2016 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | The alteration of serine transporter activity in a cell line model of amyotrophic lateral sclerosis (ALS) | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.bbrc.2016.12.178 | - |
dc.identifier.scopusid | 2-s2.0-85008452889 | - |
dc.identifier.wosid | 000397259000021 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.483, no.1, pp 135 - 141 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 483 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 135 | - |
dc.citation.endPage | 141 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | AMINO-ACID TRANSPORTER | - |
dc.subject.keywordPlus | HIGH-AFFINITY | - |
dc.subject.keywordPlus | PRIMARY CULTURE | - |
dc.subject.keywordPlus | MOTOR-NEURONS | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | RACEMASE | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | ASC-1 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | OXIDASE | - |
dc.subject.keywordAuthor | Amyotrophic lateral sclerosis (ALS) | - |
dc.subject.keywordAuthor | Motor neuron disease | - |
dc.subject.keywordAuthor | D-Serine | - |
dc.subject.keywordAuthor | L-Serine | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/abs/pii/S0006291X16322501?via%3Dihub | - |
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