Interleukin-32 alpha induces migration of human melanoma cells through downregulation of E-cadherin
DC Field | Value | Language |
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dc.contributor.author | Lee, Joohyun | - |
dc.contributor.author | Kim, Kyung Eun | - |
dc.contributor.author | Cheon, Soyoung | - |
dc.contributor.author | Song, Ju Han | - |
dc.contributor.author | Houh, Younkyung | - |
dc.contributor.author | Kim, Tae Sung | - |
dc.contributor.author | Gil, Minchan | - |
dc.contributor.author | Lee, Kyung Jin | - |
dc.contributor.author | Kim, Seonghan | - |
dc.contributor.author | Kim, Daejin | - |
dc.contributor.author | Hur, Dae Young | - |
dc.contributor.author | Yang, Yoolhee | - |
dc.contributor.author | Bang, Sa Ik | - |
dc.contributor.author | Park, Hyun Jeong | - |
dc.contributor.author | Cho, Daeho | - |
dc.date.available | 2021-02-22T11:24:11Z | - |
dc.date.issued | 2016-10 | - |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/9393 | - |
dc.description.abstract | Interleukin (IL)-32 alpha, the shortest isoform of proinflammatory cytokine IL32, is associated with various inflammatory diseases and cancers. However, its involvement in human melanoma is not understood. To determine the effect of IL-32 alpha in melanoma, IL-32 alpha levels were examined in human melanoma cell lines that exhibit different migratory abilities. IL-32 alpha levels were higher in human melanoma cell lines with more migratory ability. An IL-32 alpha-overexpressing G361 human melanoma cell line was generated to investigate the effect of IL-32 alpha on melanoma migration. IL-32 alpha-overexpressing G361 cells (G361-IL-32 alpha) exhibit an increased migratory ability compared to vector control cells (G361-vector). To identify factors involved in IL-32 alpha-induced migration, we compared expression of E-cadherin in G361-vector and G361-IL-32 alpha cells. We observed decreased levels of E-cadherin in G361-IL-32 alpha cells, resulting in F-actin polymerization. To further investigate signaling pathways related to IL-32 alpha-induced migration, we treated G361-vector and G361-IL-32 alpha cells with PD98059, a selective MEK inhibitor. Inhibition of Erk1/2 by PD98059 restored E-cadherin expression and decreased IL-32 alpha-induced migration. In addition, cell invasiveness of G361-IL-32 alpha cells was tested using an in vivo lung metastasis model. As results, lung metastasis was significantly increased by IL-32 alpha overexpression. Taken together, these data indicate that IL-32 alpha induced human melanoma migration via Erk1/2 activation, which repressed E-cadherin expression. Our findings suggest that IL-32 alpha is a novel regulator of migration in melanoma. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Impact Journals | - |
dc.title | Interleukin-32 alpha induces migration of human melanoma cells through downregulation of E-cadherin | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.18632/oncotarget.11669 | - |
dc.identifier.scopusid | 2-s2.0-84994176938 | - |
dc.identifier.wosid | 000387281000099 | - |
dc.identifier.bibliographicCitation | Oncotarget, v.7, no.40, pp 65825 - 65836 | - |
dc.citation.title | Oncotarget | - |
dc.citation.volume | 7 | - |
dc.citation.number | 40 | - |
dc.citation.startPage | 65825 | - |
dc.citation.endPage | 65836 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | GASTRIC-CANCER | - |
dc.subject.keywordPlus | RHEUMATOID-ARTHRITIS | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | IL-32 | - |
dc.subject.keywordPlus | METASTASIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INVASION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordAuthor | interleukin-32 | - |
dc.subject.keywordAuthor | melanoma | - |
dc.subject.keywordAuthor | migration | - |
dc.subject.keywordAuthor | Erk1/2 | - |
dc.subject.keywordAuthor | E-cadherin | - |
dc.identifier.url | https://www.oncotarget.com/article/11669/text/ | - |
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