Kazinol B from Broussonetia kazinoki improves insulin sensitivity via Akt and AMPK activation in 3T3-L1 adipocytes
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyejin | - |
dc.contributor.author | Li, Hua | - |
dc.contributor.author | Jeong, Ji Hye | - |
dc.contributor.author | Noh, Minsoo | - |
dc.contributor.author | Ryu, Jae-Ha | - |
dc.date.available | 2021-02-22T11:26:29Z | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 0367-326X | - |
dc.identifier.issn | 1873-6971 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/9719 | - |
dc.description.abstract | In this study, we evaluated the insulin-sensitizing effect of flavans purified from Broussonetia kazinoki Siebold (BK) on 3T3-L1 adipocytes. Among the tested compounds, kazinol B enhanced intracellular lipid accumulation, gene expression of proliferator-activated receptor gamma (PPAR gamma) and CCAAT/enhancer binding protein-alpha (C/EBP alpha), and consistently induced PPAR gamma transcriptional activation. To further investigate the insulin-sensitizing effect of kazinol B, we measured glucose analogue uptake by fully differentiated adipocytes and myotubes. Kazinol B increased 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) uptake by cells by upregulating the gene expression and translocation of glucose transporter 4 (GLUT-4) into the plasma membrane in adipocytes. Kazinol B stimulated the gene expression and secretion of adiponectin, which is associated with a low risk of types 1 and 2 diabetes mellitus. We also suggested the mechanism of the antidiabetic effect of kazinol B by assaying Akt and AMP-activated protein kinase (AMPK) phosphorylation. In conclusion, kazinol B isolated from BK improved insulin sensitivity by enhancing glucose uptake via the insulin-Akt signaling pathway and AMPK activation. These results suggest that kazinol B might be a therapeutic candidate for diabetes mellitus. (C) 2016 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Kazinol B from Broussonetia kazinoki improves insulin sensitivity via Akt and AMPK activation in 3T3-L1 adipocytes | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1016/j.fitote.2016.05.006 | - |
dc.identifier.scopusid | 2-s2.0-84974577912 | - |
dc.identifier.wosid | 000380865400012 | - |
dc.identifier.bibliographicCitation | FITOTERAPIA, v.112, pp 90 - 96 | - |
dc.citation.title | FITOTERAPIA | - |
dc.citation.volume | 112 | - |
dc.citation.startPage | 90 | - |
dc.citation.endPage | 96 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | PPAR-GAMMA AGONISTS | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | GLUCOSE-UPTAKE | - |
dc.subject.keywordPlus | GLUT4 TRANSLOCATION | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | ADIPONECTIN | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | Broussonetia kazinoki | - |
dc.subject.keywordAuthor | Diabetes mellitus | - |
dc.subject.keywordAuthor | Glucose uptake | - |
dc.subject.keywordAuthor | 3T3-L1 | - |
dc.subject.keywordAuthor | AMPK | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/abs/pii/S0367326X16301149?via%3Dihub | - |
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