A novel miR-34a target, protein kinase D1, stimulates cancer stemness and drug resistance through GSK3/beta-catenin signaling in breast cancer
DC Field | Value | Language |
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dc.contributor.author | Kim, Do Yeon | - |
dc.contributor.author | Park, Eun Young | - |
dc.contributor.author | Chang, EunSun | - |
dc.contributor.author | Kang, Hyeok-Gu | - |
dc.contributor.author | Koo, Yoonjin | - |
dc.contributor.author | Lee, Eun Ji | - |
dc.contributor.author | Ko, Je Yeong | - |
dc.contributor.author | Kong, Hyun Kyung | - |
dc.contributor.author | Chun, Kyung-Hee | - |
dc.contributor.author | Park, Jong Hoon | - |
dc.date.available | 2021-02-22T11:28:04Z | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/9905 | - |
dc.description.abstract | One of the properties of human breast cancer cells is cancer stemness, which is characterized by self-renewal capability and drug resistance. Protein kinase D1 (PRKD1) functions as a key regulator of many cellular processes and is downregulated in invasive breast cancer cells. In this study, we found that PRKD1 was upregulated in MCF-7-ADR human breast cancer cells characterized by drug resistance. Additionally, we discovered that PRKD1 expression was negatively regulated by miR-34a binding to the PRKD1 3'-UTR. PRKD1 expression increased following performance of a tumorsphere formation assay in MCF-7-ADR cells. We also found that reduction of PRKD1 by ectopic miR-34a expression or PRKD1 siRNA treatment resulted in suppressed self-renewal ability in breast cancer stem cells. Furthermore, we confirmed that the PRKD1 inhibitor CRT0066101 reduced phosphorylated PKD/PKC mu, leading to suppression of breast cancer stemness through GSK3/beta-catenin signaling. PRKD1 inhibition also influenced apoptosis initiation in MCF-7-ADR cells. Tumors from nude mice treated with miR-34a or CRT0066101 showed suppressed tumor growth, proliferation, and induced apoptosis. These results provide evidence that regulation of PRKD1, a novel miR-34a target, contributes to overcoming cancer stemness and drug resistance in human breast cancer. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | IMPACT JOURNALS LLC | - |
dc.title | A novel miR-34a target, protein kinase D1, stimulates cancer stemness and drug resistance through GSK3/beta-catenin signaling in breast cancer | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.18632/oncotarget.7443 | - |
dc.identifier.scopusid | 2-s2.0-84962854206 | - |
dc.identifier.wosid | 000375687200113 | - |
dc.identifier.bibliographicCitation | ONCOTARGET, v.7, no.12, pp 14791 - 14802 | - |
dc.citation.title | ONCOTARGET | - |
dc.citation.volume | 7 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 14791 | - |
dc.citation.endPage | 14802 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | G(Q)-COUPLED RECEPTORS | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | MICRORNA | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PROTEIN-KINASE-D1 | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INVASION | - |
dc.subject.keywordPlus | TUMORS | - |
dc.subject.keywordAuthor | miR-34a | - |
dc.subject.keywordAuthor | PRKD1 | - |
dc.subject.keywordAuthor | beta-catenin signaling | - |
dc.subject.keywordAuthor | cancer stemness | - |
dc.subject.keywordAuthor | drug resistance | - |
dc.identifier.url | https://www.oncotarget.com/article/7443/text/ | - |
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