Kazinol-E is a specific inhibitor of ERK that suppresses the enrichment of a breast cancer stem-like cell population
DC Field | Value | Language |
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dc.contributor.author | Jung, Yu-Chae | - |
dc.contributor.author | Han, Seula | - |
dc.contributor.author | Hua, Li | - |
dc.contributor.author | Ahn, Yeon-Hwa | - |
dc.contributor.author | Cho, Hyewon | - |
dc.contributor.author | Lee, Cheol-Jung | - |
dc.contributor.author | Lee, Hani | - |
dc.contributor.author | Cho, Yong-Yeon | - |
dc.contributor.author | Ryu, Jae-Ha | - |
dc.contributor.author | Jeon, Raok | - |
dc.contributor.author | Kim, Woo-Young | - |
dc.date.available | 2021-02-22T11:29:53Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/9943 | - |
dc.description.abstract | Growing evidence shows that cancer stem-like cells (CSLCs) contribute to breast cancer recurrence and to its resistance to conventional therapies. The extracellular signal-regulated kinase (ERIC) signaling pathway is a major determinant in the control of diverse cellular processes, including the maintenance of CSLCs. In this study, we found that Kazinol-E, an antioxidant flavan from Broussonetia kazinoki, decreased the CSLC population of a breast cancer cell line, MCF7. The CSLC population, characterized by CD44 high/CD24 low expression or by high Aldehyde dehydrogenase 1 activity, was decreased by a concentration of Kazinol-E that did not affect the growth of bulk-cultured MCF7 cells. Kazinol-E did not decrease EGF-induced ERIC phosphorylation in CSLCs, but did block the phosphorylation of an ERK substrate, p90RSK2, at Thr359/Ser363. We further demonstrated that EGF-induced ERK activity was blocked by Kazinol-E in a wild-type K-Ras-expressing non-small cell lung cancer cell line H226B. An in vitro kinase assay with purified ERK1 and p90RSK2 as its substrate demonstrated a direct inhibition of ERK activity by Kazinol E. Additionally, a the molecular docking study provided putative binding modes of Kazinol-E into the ATP binding pocket of ERK1 Collectively, these results suggest that Kazinol-E is a direct inhibitor of ERK1, and more studies are warranted to develop this reagent for therapeutic breast CSLC targeting. (C) 2016 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Kazinol-E is a specific inhibitor of ERK that suppresses the enrichment of a breast cancer stem-like cell population | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.bbrc.2016.01.066 | - |
dc.identifier.scopusid | 2-s2.0-84955457393 | - |
dc.identifier.wosid | 000370581300009 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.470, no.2, pp 294 - 299 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 470 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 294 | - |
dc.citation.endPage | 299 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | BROUSSONETIA-KAZINOKI | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | NOTCH | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | HETEROGENEITY | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | Breast cancer stem cell | - |
dc.subject.keywordAuthor | Extracellular signal-regulated kinase (ERK) pathway | - |
dc.subject.keywordAuthor | Kazinol-E | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/abs/pii/S0006291X16300663?via%3Dihub | - |
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