Antitumor Activity of HM781-36B, Alone or in Combination with Chemotherapeutic Agents, in Colorectal Cancer Cells
DC Field | Value | Language |
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dc.contributor.author | 강미현 | - |
dc.contributor.author | 문승욱 | - |
dc.contributor.author | 성지혜 | - |
dc.contributor.author | 김진원 | - |
dc.contributor.author | 이근욱 | - |
dc.contributor.author | 이혜승 | - |
dc.contributor.author | 이종석 | - |
dc.contributor.author | 김지현 | - |
dc.date.available | 2021-02-22T11:30:01Z | - |
dc.date.issued | 2016-01 | - |
dc.identifier.issn | 1598-2998 | - |
dc.identifier.issn | 2005-9256 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/9958 | - |
dc.description.abstract | Purpose HM781-36B is a novel and irreversible pan-human epidermal growth factor receptor (HER) inhibitor with TEC cytoplasmic kinase inhibition. The aim of this study is to evaluate the antitumor activity and mechanism of action for HM781-36B in colorectal cancer (CRC) cell lines. Materials and Methods The CRC cell lines were exposed to HM781-36B and/or oxaliplatin (L-OHP), 5-fluorouracil (5-FU), SN-38. The cell viability was examined by Cell Titer-Glo luminescent cell viability assay kit. Change in the cell cycle and protein expression was determined by flow cytometry and immunoblot analysis, respectively. Synergism between two drugs was evaluated by the com- bination index. Results The addition of HM781-36B induced potent growth inhibition in both DiFi cells with epider- mal growth factor receptor (EGFR) overexpression and SNU-175 cells (IC50, 0.003 µM and 0.005 µM, respectively). Furthermore, HM781-36B induced G1 arrest of the cell cycle and apoptosis, and reduced the levels of HER family and downstream signaling molecules, pERK and pAKT, as well as nonreceptor/cytoplasmic tyrosine kinase, BMX. The combination of HM781-36B with 5-FU, L-OHP, or SN-38 showed an additive or synergistic effect in most CRC cells. Conclusion These findings suggest the potential roles of HM781-36B as the treatment for EGFR-over- expressing colon cancer, singly or in combination with chemotherapeutic agents. The role of BMX expression as a marker of response to HM781-36B should be further explored. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 대한암학회 | - |
dc.title | Antitumor Activity of HM781-36B, Alone or in Combination with Chemotherapeutic Agents, in Colorectal Cancer Cells | - |
dc.title.alternative | Antitumor Activity of HM781-36B, Alone or in Combination with Chemotherapeutic Agents, in Colorectal Cancer Cells | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.4143/crt.2014.260 | - |
dc.identifier.scopusid | 2-s2.0-84957544485 | - |
dc.identifier.wosid | 000368215200041 | - |
dc.identifier.bibliographicCitation | Cancer Research and Treatment, v.48, no.1, pp 355 - 364 | - |
dc.citation.title | Cancer Research and Treatment | - |
dc.citation.volume | 48 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 355 | - |
dc.citation.endPage | 364 | - |
dc.identifier.kciid | ART002074292 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | EGFR INHIBITORS | - |
dc.subject.keywordPlus | PHASE-II | - |
dc.subject.keywordPlus | CETUXIMAB | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | LUNG | - |
dc.subject.keywordAuthor | HM781-36B | - |
dc.subject.keywordAuthor | Colorectal neoplasms | - |
dc.subject.keywordAuthor | Epidermal growth factor receptor-neu receptor | - |
dc.subject.keywordAuthor | BMX | - |
dc.identifier.url | https://www.e-crt.org/journal/view.php?doi=10.4143/crt.2014.260 | - |
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