Effects of Bisphosphonates on Glucose Transport in a Conditionally Immortalized Rat Retinal Capillary Endothelial Cell Line (TR-iBRB Cells)
DC Field | Value | Language |
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dc.contributor.author | Lee, Na-Young | - |
dc.contributor.author | Park, Hyun-Joo | - |
dc.contributor.author | Kang, Young-Sook | - |
dc.date.available | 2021-02-22T11:30:11Z | - |
dc.date.issued | 2016-01 | - |
dc.identifier.issn | 1976-9148 | - |
dc.identifier.issn | 2005-4483 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/9972 | - |
dc.description.abstract | The objective of the present study was to elucidate the effect of bisphosphonates, anti-osteoporosis agents, on glucose uptake in retinal capillary endothelial cells under normal and high glucose conditions. The change of glucose uptake by pre-treatment of bisphosphonates at the inner blood-retinal barrier (iBRB) was determined by measuring cellular uptake of [H-3]3-O-methyl glucose (3-OMG) using a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB cells) under normal and high glucose conditions. [H-3]3-OMG uptake was inhibited by simultaneous treatment of unlabeled D-glucose and 3-OMG as well as glucose transport inhibitor, cytochalasin B. On the other hand, simultaneous treatment of alendronate or pamidronate had no significant inhibitory effect on [H-3]3-OMG uptake by TR-iBRB cells. Under high glucose condition of TR-iBRB cells, [H-3]3-OMG uptake was increased at 48 h. However, [H-3]3-OMG uptake was decreased significantly by pre-treatment of alendronate or pannidronate compared with the values for normal and high glucose conditions. Moreover, geranylgeraniol (GGOH), a mevalonate pathway intermediate, increased the uptake of [H-3]3-OMG reduced by bisphosphonates pre-treatment. But, pre-treatment of histamine did not show significant inhibition of [H-3]3-OMG uptake. The glucose uptake may be down regulated by inhibiting the mevalonate pathway with pre-treatment of bisphosphonates in TR-iBRB cells at high glucose condition. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC APPLIED PHARMACOLOGY | - |
dc.title | Effects of Bisphosphonates on Glucose Transport in a Conditionally Immortalized Rat Retinal Capillary Endothelial Cell Line (TR-iBRB Cells) | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.4062/biomolther.2015.183 | - |
dc.identifier.scopusid | 2-s2.0-84952685446 | - |
dc.identifier.wosid | 000367640100013 | - |
dc.identifier.bibliographicCitation | BIOMOLECULES & THERAPEUTICS, v.24, no.1, pp 94 - 98 | - |
dc.citation.title | BIOMOLECULES & THERAPEUTICS | - |
dc.citation.volume | 24 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 94 | - |
dc.citation.endPage | 98 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002060607 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | MEVALONATE PATHWAY | - |
dc.subject.keywordPlus | CHOROIDAL NEOVASCULARIZATION | - |
dc.subject.keywordPlus | TAURINE TRANSPORT | - |
dc.subject.keywordPlus | BOVINE RETINA | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | BARRIER | - |
dc.subject.keywordPlus | PERMEABILITY | - |
dc.subject.keywordPlus | RETINOPATHY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | HISTAMINE | - |
dc.subject.keywordAuthor | Glucose uptake | - |
dc.subject.keywordAuthor | Bisphosphonates | - |
dc.subject.keywordAuthor | Inner blood-retinal barrier | - |
dc.subject.keywordAuthor | Retinal capillary endothelial cells | - |
dc.subject.keywordAuthor | Mevalonate pathway | - |
dc.identifier.url | http://www.biomolther.org/journal/view.html?volume=24&number=1&spage=94&year=2016 | - |
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