Structural Characterization for N-Terminal Domain of Caveolin-1
- Authors
- 김종민; 신재영; 박헌영
- Issue Date
- Sep-2003
- Publisher
- 한국통합생물학회
- Keywords
- Caveolin-1Protein foldingAssociationStabilityCaveolaeCaveolin-1 is a principal protein in the plasma membrane microdomains calledcaveolae. Caveolae play an important role in the transcytosis and pinocytosis.Therefore; caveolin-1 is most likely to wor
- Citation
- Animal Cells and Systems, v.7, no.3, pp 207 - 211
- Pages
- 5
- Journal Title
- Animal Cells and Systems
- Volume
- 7
- Number
- 3
- Start Page
- 207
- End Page
- 211
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/10271
- ISSN
- 1976-8354
2151-2485
- Abstract
- Caveolin-1 is a principal protein in the plasma membrane microdomains called caveolae. Caveolae play an important role in the transcytosis and pinocytosis. Therefore, caveolin-1 is most likely to work for the membrane dynamic events. In addition, caveolin-1 interacts with various signaling molecules. Although caveolin-1 possesses a variety of physiological functions, its structural properties were little construed. Here we analyzed the structural dynamics of the N-terminal caveolin-1 (residues 1-101), in order to better understand the structural properties in terms of its versatile functionality. We first analyzed its oligomeric form using GST-fused N-terminal domain, revealing that it equilibrates between a dimer and monomers in a concentration-dependent manner. The N-terminal domain of caveolin-1 was previously found to form a heptamer, so that our data suggest the dimeric form as an intermediate structure for the heptamer formation. Then, we obtained the folding profile, which indicated that GH2O is about 0.5 ± 0.03 kcal / mol. The stability of N-terminal domain is relatively low, indicating that N-terminal domain may not be crystalline. Conclusively, the dynamic and flexible structure of N-terminal domain appears more favorable to maintain the versatile functions of caveolin-1.
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