Effects of cooking methods and chemical tenderizers on survival of Escherichia coli O157:H7 in ground beef patties

Abstract

This study evaluated chemical tenderizers and cooking methods to inactivate Escherichia coli O157:H7 in ground beef patties (model system for non-intact beef). Ground beef was inoculated with E. coli O157:H7 and mixed with (i) nothing (control), (ii) calcium chloride (CC) and flavoring agents (FA), (iii) CC, FA, and acetic acid (AA), (iv) sodium chloride (SC), sodium tripolyphosphate (ST), and potassium lactate (PL), and (v) the combination of SC, ST, PL, and AA. Patties were stored in aerobic or vacuum bags at -20, 4, and 12 degrees C. Samples were grilled, broiled, or pan-fried to 60 or 65 degrees C. Total bacterial and E. coli O157:H7 populations remained unchanged during storage. Broiling was more effective in reducing E. coli O157:H7 than grilling and pan-frying, and acidified tenderizers reduced E. coli O157:H7 more than non-acidified tenderizers in broiling. Higher reductions were observed at 65 degrees C than 60 degrees C in broiled and grilled samples. These results indicate that acidified tenderizers and broiling may be useful in non-intact beef safety. (c) 2013 Elsevier Ltd. All rights reserved.