글루타알데하이드에 의한 키토산 부직포에 트립신 고정화Immobilization of Trypsin on Chitosan Nonwoven Using Glutaraldehyde
- Other Titles
- Immobilization of Trypsin on Chitosan Nonwoven Using Glutaraldehyde
- Authors
- 김정수; 이소희; 송화순
- Issue Date
- Oct-2013
- Publisher
- 한국의류학회
- Keywords
- 효소; 고정화; 키토산; 트립신; 글루타알데하이드; Enzyme; Immobilization; Chitosan; Trypsin; Glutaraldehyde
- Citation
- 한국의류학회지, v.37, no.7, pp 852 - 863
- Pages
- 12
- Journal Title
- 한국의류학회지
- Volume
- 37
- Number
- 7
- Start Page
- 852
- End Page
- 863
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/11506
- DOI
- 10.5850/JKSCT.2013.37.7.852
- ISSN
- 1225-1151
2234-0793
- Abstract
- We investigate the immobilization of trypsin on chitosan nonwoven using glutaraldehyde (GA). The conditions for trypsin on chitosan nonwoven and GA cross-linking were optimized depending on different conditions. The order of GA cross-linking was determined by the activity of immobilized trypsin. The characteristics of chitosan nonwoven were examined by Fourier-transform infrared (FT-IR) and surface morphology analyses (SEM). Results showed that the optimal treatment conditions for trypsin on chitosan nonwoven were as follows: pH 8.5; temperature 37℃; trypsin concentration 15% (o.w.f); and treatment time 60 min. Those for GA cross-linking were: pH 10.0; GA concentration 3% (v/v); and treatment time 120 min. FT-IR analysis showed that GA was cross-linked on chitosan nonwoven. The SEM analysis also showed that trypsin was immobilized on chitosan nonwoven.
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