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Micropillar/Microwell Chip Assessment for Detoxification of Bisphenol A with Korean Pear (Pyrus pyrifolia)

Authors
Lee, Dong WooLee, Moo-YealKoh, SukkilYang, Mihi
Issue Date
Oct-2020
Publisher
MDPI
Citation
MICROMACHINES, v.11, no.10
Journal Title
MICROMACHINES
Volume
11
Number
10
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/1150
DOI
10.3390/mi11100922
ISSN
2072-666X
2072-666X
Abstract
A micropillar/microwell chip platform with 3D cultured liver cells has been used for HTP screening of hepatotoxicity of bisphenol A (BPA), an endocrine-disrupting chemical. We previously found the hepatotoxicity of BPA is alleviated by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase 2 (ALDH2). In this study, we have tested potential BPA detoxification with Korean pear (Pyrus pyrifolia) extract, stimulators of ADH and ALDH, as well as arbutin, a reference compound in the pears, on the micropillar/microwell chip platform with human liver cells. Surprisingly, the toxicity of BPA was reduced in the presence of Korean pear extract, indicated by significantly increased IC50 values. The IC50 value of BPA with Korean pear extract tested against HepG2 cells was shifted from 151 to 451 mu M, whereas those tested against Hep3B cells was shifted from 110 to 204 mu M. Among the tested various concentrations, 1.25, 2.5, and 5 mg/mL of the extract significantly reduced BPA toxicity (Ps < 0.05). However, there was no such detoxification effects with arbutin. This result was supported by changes in protein levels of ADH in the liver cells.
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