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Detection of Helicobacter pylori DNA in preliminary stage gastric cancer cells
- Authors
- Ly, Suw Young; Yoo, So Dam; Chun, Seung Ku
- Issue Date
- Apr-2012
- Publisher
- ELSEVIER
- Keywords
- Gastric cancer; Helicobacter pylori; voltammetry
- Citation
- PATHOLOGY, v.44, no.3, pp 251 - 254
- Pages
- 4
- Journal Title
- PATHOLOGY
- Volume
- 44
- Number
- 3
- Start Page
- 251
- End Page
- 254
- ISSN
- 0031-3025
1465-3931
- Abstract
- Aims: Helicobacter pylori (HP) DNA was assayed using a newly fabricated voltammetric handheld circuit equipped with an interfaced graphite pencil electrode (PE) sensor. Methods: Under optimum analytical parameters, the square-wave (SW) stripping detection range attained was 0.1-0.7 mg/L. Results: A relative standard deviation of 1.68% (RSD, n = 5) was observed at a 3.2 mg/L HP DNA within a 90s accumulation time. Under these conditions, the analytical detection limit approached 0.07 mg/L. Conclusions: The developed methods can be applied to HP assays of preliminary stage gastric cancer patient tissues.
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