[5-(3-Indol-1-ylpropoxy)-1H-indol-3-yl] Acetic Acid Enhances Adipocyte Differentiation and Glucose Uptake in 3T3-L1 Cells
- Authors
- Lee, I.; Yu, J.; Yoon, Y.; Gim, H. J.; Lee, S. M.; Park, J. W.; Jeon, R.; Park, B. H.
- Issue Date
- 1-Jul-2011
- Publisher
- ASIAN NETWORK SCIENTIFIC INFORMATION-ANSINET
- Keywords
- PPAR gamma; adipogenesis; glucose uptake; insulin; adipocyte; rosiglitazone
- Citation
- INTERNATIONAL JOURNAL OF PHARMACOLOGY, v.7, no.5, pp 647 - 652
- Pages
- 6
- Journal Title
- INTERNATIONAL JOURNAL OF PHARMACOLOGY
- Volume
- 7
- Number
- 5
- Start Page
- 647
- End Page
- 652
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/12539
- DOI
- 10.3923/ijp.2011.647.652
- ISSN
- 1811-7775
1812-5700
- Abstract
- The nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR gamma) plays an important role in adipocyte differentiation and is the target for anti-diabetic drugs known as thiazolidinediones. Here, we synthesized and characterized a new PPAR gamma agonist, SPA0432. COS-7 cells treated with SPA0432 showed significantly increased PPAR gamma transcriptional activity compared to that of vehicle-treated cells. However, its efficacy was less than that of rosiglitazone. Using a standard differentiation protocol, SPA0432 effectively enhanced differentiation of 3T3-L1 preadipocytes as evidenced by increased lipid droplet formation and triglyceride accumulation. Real-time RT-PCR analysis indicated that SPA0432 significantly increased the expressions of adipogenesis-related genes, CAAT/enhancer binding protein alpha, PPAR gamma, fatty acid synthase, aP2 and lipoprotein lipase and significantly decreased the expression of Pref-1, a preadipocyte marker. Moreover, SPA0432 increased insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes. These results suggest that SAP0432 may exert beneficial effects against insulin resistance through its ability to promote adipocyte differentiation and insulin-stimulated glucose uptake.
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