Cloning and characterization of bovine interleukin-32 beta isoform
- Authors
- Jaekal, Jun; Jhun, Hyunjhung; Hong, Jaewoo; Park, Seungyoung; Lee, Joongbok; Yoon, Doyoung; Lee, Siyoung; Her, Erk; Yang, Young; Rho, Gyujin; Kim, Soohyun
- Issue Date
- Sep-2010
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Cytokine; Recombinant protein; Inflammation; Affinity chromatography; Peripheral blood mononuclear cells (PBMC)
- Citation
- VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, v.137, no.1-2, pp 166 - 171
- Pages
- 6
- Journal Title
- VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
- Volume
- 137
- Number
- 1-2
- Start Page
- 166
- End Page
- 171
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13126
- DOI
- 10.1016/j.vetimm.2010.04.019
- ISSN
- 0165-2427
1873-2534
- Abstract
- Interleukin-32 (IL-32) is a new cytokine produced mainly by T-cells, natural killer cells, and epithelial cells after stimulation with IL-2, IL-12 plus IL-18, and IFN gamma, respectively. IL-32 induces various proinflammatory cytokines such as TNF alpha, IL-1 beta. IL-6, and IL-8 in monocytes and macrophages In this study, we cloned bovine IL-32 cDNA from peripheral blood mononuclear cells (PBMC) of a Holstein (Bos Taurus). The bovine IL-32 cDNA has an entire open reading frame, encoding 171 amino acid residues and the deduced amino acid sequence has 27 5% identity with human IL-32 beta isoform. Recombinant bovine IL-32 protein was produced in E. coli and its molecular weight was approximately 26 kDa The biological activity of the bovine IL-32 was examined for cytokine induction using human monocytic THP-1 cells and bovine PBMC The THP-1 cells responded to stimulation by recombinant bovine IL-32 and produced IL-8 Stimulation by recombinant bovine IL-32 induced mRNA for TNFa and IL-6 in bovine PBMC suggesting conservation of the biological activity and function in cattle (C) 2010 Elsevier B V All rights reserved
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