Expression of Th1 and Th2 cytokine-associated transcription factors, T-bet and GATA-3, in peripheral blood mononuclear cells and skin lesions of patients with psoriasis vulgaris
- Authors
- Huh, Sungran; Jung, Eunsun; Lee, Jienny; Roh, Kyungbaeg; Kim, Ju-Duck; Lee, Jongsung; Park, Deokhoon
- Issue Date
- Sep-2010
- Publisher
- SPRINGER
- Keywords
- Melanogenesis; Propafenone; Tyrosinase; MITF
- Citation
- ARCHIVES OF DERMATOLOGICAL RESEARCH, v.302, no.7, pp 561 - 565
- Pages
- 5
- Journal Title
- ARCHIVES OF DERMATOLOGICAL RESEARCH
- Volume
- 302
- Number
- 7
- Start Page
- 561
- End Page
- 565
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13140
- DOI
- 10.1007/s00403-010-1059-y
- ISSN
- 0340-3696
1432-069X
- Abstract
- Psoriasis is considered as a T cell-mediated autoimmune disease, and the Th1 response has been established as the major immune agent in its pathomechanisms. The relative expression of Th1 and Th2 transcription factors, T-bet and GATA-3, resulting in a swing in the Th1/Th2 pendulum, has been implicated in a number of immunological diseases. However, their expression and correlation with psoriasis has not yet been studied. Our aim was to evaluate the expression of T-bet and GATA-3 in psoriatic patients and determine their correlation with serum levels of IFN-gamma and IL-4. Sera, peripheral blood mononuclear cells (PBMCs) and skin lesions were taken from 23 patients with psoriasis vulgaris. Serum levels of IFN-gamma and IL-4 were measured by ELISA. T-bet and GATA-3 mRNA expression in PBMCs was analyzed by RT-PCR. Lesional expression and distribution of CD4, CD8, T-bet and GATA-3 were assessed by immunohistochemistry. Blood and skin samples of healthy individuals served as controls. A markedly higher IFN-gamma and lower IL-4 concentration in the serum of psoriatic patients was found. A significantly higher expression of T-bet mRNA and a lower expression of GATA-3 mRNA in PBMCs, and consequently, a much higher T-bet/GATA-3 ratio in patients than in controls were shown. T-bet mRNA expression was strongly correlated with serum IFN-gamma secretion in patients; furthermore, the correlation between T-bet/GATA-3 ratio and IFN-gamma/IL-4 ratio was revealed. We also observed a significant increase in CD4+ cells and T-bet positive cells in psoriatic lesions. These results suggested that T-bet and GATA-3 might be regulator genes for psoriasis via the Th1/Th2 balance, and the Th1-specific transcription factor, T-bet, may play an important role in the development and maintenance of psoriasis.
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