Detailed Information

Cited 0 time in webofscience Cited 51 time in scopus
Metadata Downloads

Pterostilbene, a natural dimethylated analog of resveratrol, inhibits rat aortic vascular smooth muscle cell proliferation by blocking Akt-dependent pathway

Authors
Park, Eun-SeokLim, YongHong, Jin-TaeYoo, Hwan-SooLee, Chong-KilPyo, Myoung-YunYun, Yeo-Pyo
Issue Date
Jul-2010
Publisher
ELSEVIER SCIENCE INC
Keywords
Pterostilbene; Vascular smooth muscle cell; Resveratrol and proliferation; Akt kinase
Citation
VASCULAR PHARMACOLOGY, v.53, no.1-2, pp.61 - 67
Journal Title
VASCULAR PHARMACOLOGY
Volume
53
Number
1-2
Start Page
61
End Page
67
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13174
DOI
10.1016/j.vph.2010.04.001
ISSN
1537-1891
Abstract
Vascular smooth muscle cells (VSMCs) are the main cellular component in the arterial wall, and abnormal proliferation of VSMCs plays a central role in the pathogenesis of atherosclerosis and restenosis after angioplasty, and possibly in the development of hypertension. Pterostilbene, a natural dimethylated analog of resveratrol, is known to have diverse pharmacological activities including anti-cancer, anti-inflammation and anti-oxidant activities. The present study was designed to investigate the effects of pterostilbene on platelet-derived growth factor (PDGF)-BB-induced VSMCs proliferation as well as the molecular mechanisms of the antiproliferative effects. The cell growth of VSMCs was determined by cell counting and [H-3]thymidine incorporation assays. Pterostilbene significantly inhibited the DNA synthesis and proliferation of PDGF-BB-stimulated VSMCS in a concentration-dependent manner. The inhibition percentages of pterostilbene at 1,3 and 5 mu M to VSMCs proliferation were 68.5, 80.7 and 94.6%, respectively. The DNA synthesis of pterostilbene at 1, 3 and 5 mu M in VSMCs was inhibited by 47.4, 76.7 and 100%, respectively. Pterostilbene inhibited the PDGF-BB-stimulated phosphorylation of Akt kinase. However, pterostilbene did not change the expression of extracellular signal-related kinase (ERK) 1/2, PLC gamma 1, phosphatidylinositol (PI)3 kinase and PDGF-W phosphorylation. In addition. pterostilbene down-regulated the cell cycle-related proteins including the expression of cyclin-dependent kinase (CDK) 2. cyclin E, CDK4, cyclin D1, retinoblastoma (Rb) proteins and proliferative cell nuclear antigen (PCNA). These findings suggest that the inhibition of pterostilbene to the cell proliferation and DNA synthesis of PDGF-BB-stimulated VSMCs may be mediated by the suppression of Akt kinase. Furthermore, pterostilbene may be a potential anti-proliferative agent for the treatment of atherosclerosis and angioplasty restenosis. (C) 2010 Elsevier Inc. All rights reserved.
Files in This Item
There are no files associated with this item.
Appears in
Collections
약학대학 > 약학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE