Mouse 악성흑색종에서 Lipoic Acid가 항산화 효소계에 미치는 영향

Abstract

Reactive oxygen species (ROS) has been reported to cause DNA damage, lipid peroxidation, or protein denaturation and to be related also to the various diseases such as cancer, aging, cardiovascular disease,inflammation radiation injury, etc. through the different mechanisms. Being produced in the process of natural metabolism, ROS is controlled in the cell by the antioxidative enzymes of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), etc. Belonging to the sulfur containing compound,α-lipoic acid is a physioactive substance from the mitochondria energy metabolism, and its oxidized form (disulfide bonding in a molecule) can be reduced to two SH-free radicals that are distinguished in antioxidative effect. We have measured MTT assay, enzyme activity assay, and ROS level in order to examine antioxidative effect of the lipoic acid in the B16F10 murine melenoma cell. B16F10 cells were exposed to medium containing lipoic acid for a period of 24 h. Lipoic acid increased the activities of superoxide dismutase and catalase compared to those of the control, but decresed the activity of glutathione peroxidase. Lipoic acid also reduced ROS content in a dose-dependent manner. (Cancer Prev Res 15,152-157, 2010)