Antiplatelet activity of beta-carboline alkaloids from Perganum harmala: A possible mechanism through inhibiting PLC gamma 2 phosphorylation

Abstract

Beta-carboline alkaloids including harmalol, harmaline, norharmane, harmol, harmine and harmane are important constituents of the medicinal plant, Perganum harmala L. (Zygophylaceae), which has been used in traditional medicine. In the present study, the antiplatelet activities of six beta-carboline alkaloid compounds were investigated in vitro. At a concentration of 200 mu M, these compounds have no effect on arachidonic acid (AA), thrombin- and U46619 (a thromboxane A(2) Mimic)-stimulated platelet aggregation. On the contrary, it was revealed that collagen-induced platelet aggregation could be inhibited by these compounds with different potencies (harmane and harmine were most potent, harmol had medium potency, and harmol, norharmane, harmalol and harmaline had a weak, non significant effect). indicating a selective inhibition on collagen-mediated platelet activation. Consistently, further study revealed that collage n-mediated phospholipase (PL) C gamma 2 and protein tyrosine phosphorylation, cytosolic calcium mobilization and arachidonic acid liberation were completely inhibited by harmane and harmine in a concentration-dependent manner, while the other compounds were only partially or not effective at all. Taken together, these results indicate that three of these six beta-carboline alkaloids can selectively affect collagen-induced platelet aggregation with different potencies: in particular, harmane and harmine were most potent, and their antiplatelet activities may be mediated by inhibiting PLC gamma 2 and protein tyrosine phosphorylation with sequential suppression of cytosolic calcium mobilization and arachidonic acid liberation, indicating that harmane and harmine have a potential to be developed as a novel agent for atherothrombotic diseases. (C) 2008 Published by Elsevier Inc.