AIMP1/p43 protein induces the maturation of bone marrow-derived dendritic cells with T helper type 1-polarizing ability
- Authors
- Kim, Eugene; Kim, Seung Hyun; Kim, Sunghoon; Cho, Daeho; Kim, Tae Sung
- Issue Date
- 1-Mar-2008
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Citation
- JOURNAL OF IMMUNOLOGY, v.180, no.5, pp 2894 - 2902
- Pages
- 9
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Volume
- 180
- Number
- 5
- Start Page
- 2894
- End Page
- 2902
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14301
- DOI
- 10.4049/jimmunol.180.5.2894
- ISSN
- 0022-1767
1550-6606
- Abstract
- AIMP1 (ARS-interacting multifunctional protein 1), previously known as p43, was initially identified as a factor associated with a macromolecular tRNA synthetase complex. Recently, we demonstrated that AIMP1 is also secreted and acts as a novel pleiotropic cytokine. In this study, we investigated whether AIMP1 induces the activation and maturation of murine bone marrow-derived dendritic cells (DCs). AIMP1-treated DCs exhibited up-regulated expression of cell-surface molecules, including CD40, CD86, and MHC class II. Additionally, microarray analysis and RT-PCR determinations indicated that the expression of known DC maturation genes also increased significantly following treatment with AIMP1. Treatment of DCs with AIMP1 resulted in a significant increase in IL-12 production and Ag-presenting capability, and it also stimulated the proliferation of allogeneic T cells. Importantly, AIMP1-treated DCs induced activation of Ag-specific Th type 1 (Th1) cells in vitro and in vivo. AIMP1-stimulated DCs significantly enhanced the IFN-gamma production of cocultured CD4(+) T cells. Immunization of mice with keyhole limpet hemocyanin-pulsed AIMP1 DO efficiently led to Ag-specific Th1 cell responses, as determined by flow cytometry and ELISA. The addition of a neutralizing anti-IL-12 mAb to the cell cultures that had been treated with AIMP1 resulted in the decreased production of IFN-gamma, thereby indicating that AIMP1-stimulated DCs may enhance the Th1 response through increased production of IL-12 by APCs. Taken together, these results indicate that AIMP1 protein induces the maturation and activation of DCs, which skew the immune response toward a Th1 response.
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