Levodopa의 이온토포레시스 경피전달: 올레인산 마이크로에멀젼 및 에탄올의 투과증진Iontophoretic Delivery of Levodopa: Permeation Enhancement by Oleic Acid Microemulsion and Ethanol
- Other Titles
- Iontophoretic Delivery of Levodopa: Permeation Enhancement by Oleic Acid Microemulsion and Ethanol
- Authors
- 정신애; 곽혜선; 전인구; 오승열
- Issue Date
- Dec-2008
- Publisher
- 한국약제학회
- Keywords
- Iontophoresis; levodopa; microemulsion; oleic acid; ethanol
- Citation
- Journal of Pharmaceutical Investigation, v.38, no.6, pp 373 - 380
- Pages
- 8
- Journal Title
- Journal of Pharmaceutical Investigation
- Volume
- 38
- Number
- 6
- Start Page
- 373
- End Page
- 380
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14425
- ISSN
- 2093-5552
2093-6214
- Abstract
- In order to develop optimal formulation and iontophoresis condition for the transdermal delivery of levodopa, we have evaluated the effect of two permeation enhancers, ethanol and oleic acid in microemulsion, on transdermal delivery of levodopa. In vitro flux studies were performed at 33oC, using side-by-side diffusion cell and full thickness hairless mouse skin. Current density applied was 0.4 mA/cm2 and current was off after 6 hours application. Levodopa was analysed by HPLC at 280 nm. The o/w microemulsions of oleic acid in buffer solution (pH 2.5 & 4.5) were prepared using oleic acid, Tween 80 and ethanol. The existence of microemulsion regions were investigated in pseudo-ternary phase diagrams. Contrary to our expectation, cumulative amount of levodopa transported from microemulsion (pH 2.5) for 10 hours
was similar to that from aqueous solution in all delivery methods (passive, anodal and cathodal). When pH of the microemulsion was pH 4.5, cumulative amount of levodopa transported for 10 hours increased about 40% (anodal) to 50% (cathodal), when compared to that from aqueous solution. Flux from pH 4.5 microemulsion showed higher value than that from pH 2.5 in all delivery methods. These results seem to indicate that electroosmosis plays more dominant role than electrorepulsion in the flux of levodopa at pH 2.5. The effect of ethanol on iontophoretic flux was studied using pH 2.5 phosphate buffer solution containing 3% or 5% (v/v) ethanol. Flux enhancement was observed in passive and anodal delivery as the concentration of the ethanol increased. Without ethanol, cathodal delivery showed higher flux than anodal delivery. Anodal delivery increased the cumulative amount of levodopa transported 1.6 fold by 5% ethanol after 10 hours. However, in cathodal delivery, no flux enhancement of levodopa was observed during current application and only marginal increase in cumulative amount transported after 10 hours was observed by 5% ethanol. These results seem to be related to the decrease in dielectric constant of the medium and the lipid extraction of the ethanol, which decrease the electroosmotic flow, and thus decrease the flux. Overall, the results provide important insights into the role of electroosmosis and electrorepulsion in the transport of levodopa through skin, and provide some useful informations for optimal formulation for levodopa.
- Files in This Item
-
Go to Link
- Appears in
Collections - 약학대학 > 약학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.