Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Regulation of tumor-associated macrophage (TAM) differentiation by NDRG2 expression in breast cancer cellsRegulation of tumor-associated macrophage (TAM) differentiation by NDRG2 expression in breast cancer cells

Other Titles
Regulation of tumor-associated macrophage (TAM) differentiation by NDRG2 expression in breast cancer cells
Authors
Lee, S.Lee, A.Lim, J.Lim, J.-S.
Issue Date
Feb-2022
Publisher
Korean Society for Biochemistry and Molecular Biology
Keywords
Breast cancer; Macrophage differentiation; NDRG2; Tumorassociated macrophages; Tumor microenvironment
Citation
BMB reports, v.55, no.2, pp.81 - 86
Journal Title
BMB reports
Volume
55
Number
2
Start Page
81
End Page
86
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/145871
DOI
10.5483/bmbrep.2022.55.2.114
ISSN
1976-6696
Abstract
Macrophages are a major cellular component of innate immunity and are mainly known to have phagocytic activity. In the tumor microenvironment (TME), they can be differentiated into tumor-associated macrophages (TAMs). As the most abundant immune cells in the TME, TAMs promote tumor progression by enhancing angiogenesis, suppressing T cells and increasing immunosuppressive cytokine production. N-myc downstream-regulated gene 2 (NDRG2) is a tumor suppressor gene, whose expression is downregulated in various cancers. However, the effect of NDRG2 on the differentiation of macrophages into TAMs in breast cancer remains elusive. In this study, we investigated the effect of NDRG2 expression in breast cancer cells on the differentiation of macrophages into TAMs. Compared to tumor cell-conditioned medium (TCCM) from 4T1-mock cells, TCCM from NDRG2-overexpressing 4T1 mouse breast cancer cells did not significantly change the morphology of RAW 264.7 cells. However, TCCM from 4T1-NDRG2 cells reduced the mRNA levels of TAM-related genes, including MR1, IL-10, ARG1 and iNOS, in RAW 264.7 cells. In addition, TCCM from 4T1-NDRG2 cells reduced the expression of TAM-related surface markers, such as CD206, in peritoneal macrophages (PEM). The mRNA expression of TAMrelated genes, including IL-10, YM1, FIZZ1, MR1, ARG1 and iNOS, was also downregulated by TCCM from 4T1-NDRG2 cells. Remarkably, TCCM from 4T1-NDRG2 cells reduced the expression of PD-L1 and Fra-1 as well as the production of GM-CSF, IL-10 and ROS, leading to the attenuation of T cellinhibitory activity of PEM. These data showed that compared with TCCM from 4T1-mock cells, TCCM from 4T1-NDRG2 cells suppressed the TAM differentiation and activation. Collectively, these results suggest that NDRG2 expression in breast cancer may reduce the differentiation of macrophages into TAMs in the TME. [BMB Reports 2022;55(2): 81-86].
Files in This Item
Go to Link
Appears in
Collections
이과대학 > 생명시스템학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Lim, Jong Seok photo

Lim, Jong Seok
이과대학 (생명시스템학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE