Anti-inflammatory effects of 8-hydroxy-2 '-deoxyguanosine on lipopolysaccharide-induced inflammation via Rac suppression in Balb/c mice
DC Field | Value | Language |
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dc.contributor.author | Choi, Seongwon | - |
dc.contributor.author | Choi, Hyun-Ho | - |
dc.contributor.author | Lee, Sun-Hye | - |
dc.contributor.author | Ko, Seong-Hee | - |
dc.contributor.author | You, Ho-Jin | - |
dc.contributor.author | Ye, Sang-Kyu | - |
dc.contributor.author | Chung, Myung-Hee | - |
dc.date.available | 2021-02-22T15:01:28Z | - |
dc.date.issued | 2007-12-15 | - |
dc.identifier.issn | 0891-5849 | - |
dc.identifier.issn | 1873-4596 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14592 | - |
dc.description.abstract | Recently, we observed that 8-hydroxyguanosine triphosphate and 8-hydroxy-2'-deoxyguanosine (oh(8)dG) inactivate Rac and consequently down-regulate the Rac-linked NADPH oxidase, iNOS, and Cox2. Based on these observations, we tested whether oh(8)dG has anti-inflammatory activity in vivo in lipopolysaccharide (LPS)-treated mice. LPS (1 mg/kg, ip)-treated mice exhibit marked inflammatory responses, including increases in proinflammatory cytokines (TNF-alpha, IL-6, IL-18, and IL-12p70) in serum and infiltration of neutrophils, increased translocation of NF-kappa B p50 from the cytosol to the nucleus, and phosphorylation of c-Jun in lung tissues. Mice were pretreated with oh(8)dG (up to 60 mg/kg, ip) 4 h before LPS injection, and this pretreatment dose-dependently inhibited the inflammatory responses; the inhibitions observed with 60 mg/kg oh(8)dG were statistically significant. At the same time, oh(8)dG pretreatment inactivated Rac in lung tissues. Oh(8)dG pretreatment (50 mg/kg, ip) also significantly protected against LPS-induced septic death. Furthermore, oh(8)dG was more effective than acetyl salicylic acid in inhibiting these inflammatory responses. 8-Hydroxyguanosine also had some effect but was much weaker than oh(8)dG. The effects of normal nucleosides (dG, G, and A) were negligible or not significant. These results support an anti-inflammatory activity for oh(8)dG, which could be ascribed to its Rac-inactivating action. (C) 2007 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title | Anti-inflammatory effects of 8-hydroxy-2 '-deoxyguanosine on lipopolysaccharide-induced inflammation via Rac suppression in Balb/c mice | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.freeradbiomed.2007.08.022 | - |
dc.identifier.scopusid | 2-s2.0-36248929864 | - |
dc.identifier.wosid | 000251670500004 | - |
dc.identifier.bibliographicCitation | FREE RADICAL BIOLOGY AND MEDICINE, v.43, no.12, pp 1594 - 1603 | - |
dc.citation.title | FREE RADICAL BIOLOGY AND MEDICINE | - |
dc.citation.volume | 43 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1594 | - |
dc.citation.endPage | 1603 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.subject.keywordPlus | OXIDATIVE DNA-DAMAGE | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | RESPIRATORY BURST | - |
dc.subject.keywordPlus | HUMAN NEUTROPHILS | - |
dc.subject.keywordPlus | NUCLEAR-FACTOR | - |
dc.subject.keywordPlus | POTENTIAL ROLE | - |
dc.subject.keywordPlus | NADPH OXIDASE | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordAuthor | 8-hydroxy-2 '-deoxyguanosine | - |
dc.subject.keywordAuthor | acetyl salicylic acid | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | Rac | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | lipopolysaccharde | - |
dc.subject.keywordAuthor | free radicals | - |
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