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Gene Expression Profiling of Rewarding Effect in Methamphetamine Treated Bax-deficient Mouse

Authors
Ryu, Na KyungYang, Moon HeeJung, Min SeokJeon, Jeong OkKim, Kee WonPark, Jong Hoon
Issue Date
Jul-2007
Publisher
KOREAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY
Keywords
Bax; brain; conditioned place preference; methamphetamine; microarray
Citation
BMB Reports, v.40, no.4, pp.475 - 485
Journal Title
BMB Reports
Volume
40
Number
4
Start Page
475
End Page
485
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14661
DOI
10.5483/BMBRep.2007.40.4.475
ISSN
1225-8687
Abstract
Methamphetamine is an illicit drug that is often abused and can cause neuropsychiatric and neurotoxic damage. Repeated administration of psychostimulants such as methamphetamine induces a behavioral sensitization. According to a previous study, Bax was involved in neurotoxicity by methamphetamine, but the function of Bax in rewarding effect has not yet been elucidated. Therefore, we have studied the function of Bax in a rewarding effect model. In the present study, we treated chronic methamphetamine exposure in a Bax-deficient mouse model and examined behavioral change using a conditioned place preference (CPP) test. The CPP score in Bax knockout mice was decreased compared to that of wild-type mice. Therefore, we screened for Bax-related genes that are involved in rewarding effect using microarray technology. In order to confirm microarray data, we applied the RT-PCR method to observe relative changes of Bcl2, a pro-apoptotic family gene. As a result, using our experiment microarray, we selected genes that were associated with Bax in microarray data, and eventually selected the Tgfbr2 gene. Expression of the Tgfbr2 gene was decreased by methamphetamine in Bax knockout mice, and the gene was overexpressed in Bax wild-type mice. Additionally, we confirmed that Creb, FosB, and c-Fos were related to rewarding effect and Bax using immunohistochemistry.
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