Differentiation of eosinophilic leukemia EoL-1 cells into eosinophils induced by histone deacetylase inhibitors
- Authors
- Ishihara, Kenji; Takahashi, Aki; Kaneko, Motoko; Sugeno, Hiroki; Hirasawa, Noriyasu; Hong, JangJa; Zee, OkPyo; Ohuchi, Kazuo
- Issue Date
- Mar-2007
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- EoL-1 cells; eosinophils; differentiation; histone deacetylase inhibitor
- Citation
- LIFE SCIENCES, v.80, no.13, pp 1213 - 1220
- Pages
- 8
- Journal Title
- LIFE SCIENCES
- Volume
- 80
- Number
- 13
- Start Page
- 1213
- End Page
- 1220
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14723
- DOI
- 10.1016/j.lfs.2006.12.016
- ISSN
- 0024-3205
1879-0631
- Abstract
- EoL-1 cells differentiate into eosinophils in the presence of n-butyrate, but the mechanism has remained to be elucidated. Because n-butyrate can inhibit histone deacetylases, we hypothesized that the inhibition of histone deacetylases induces the differentiation of EoL-1 cells into eosinophils. In this study, using n-butyrate and two other histone deacetylase inhibitors, apicidin and trichostatin A, we have analyzed the relationship between the inhibition of histone deacetylases and the differentiation into eosinophils in EoL-1 cells. It was demonstrated that apicidin and n-butyrate induced a continuous acetylation of histones H4 and 143, inhibited the proliferation of EoL-1 cells without attenuating the level of FIP1L1-PDGFRA mRNA, and induced the expression of markers for mature eosinophils such as integrin beta 7, CCR1, and CCR3 on EoL-1 cells, while trichostatin A evoked a transient acetylation of histones and induced no differentiation into eosinophils. These findings suggest that the continuous inhibition of histone deacetylases in EoL-1 cells induces the differentiation into mature eosinophils. (c) 2007 Elsevier Inc. All rights reserved.
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