Mitochondria-Immobilized pH-Sensitive Off-On Fluorescent Probe
- Authors
- Lee, Min Hee; Park, Nayoung; Yi, Chunsik; Han, Ji Hye; Hong, Ji Hye; Kim, Kwang Pyo; Kang, Dong Hoon; Sessler, Jonathan L.; Kang, Chulhun; Kim, Jong Seung
- Issue Date
- Oct-2014
- Publisher
- American Chemical Society
- Citation
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.136, no.40, pp 14136 - 14142
- Pages
- 7
- Journal Title
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- Volume
- 136
- Number
- 40
- Start Page
- 14136
- End Page
- 14142
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/147257
- DOI
- 10.1021/ja506301n
- ISSN
- 0002-7863
1520-5126
- Abstract
- We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off-on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reactive benzyl chloride subunit for mitochondrial fixation. It operates well in a mitochondrial environment within whole cells and displays a desirable off-on fluorescence response to mitochondrial acidification. Moreover, this probe allows for the monitoring of impaired mitochondria undergoing mitophagic elimination as the result of nutrient starvation. It thus allows for the monitoring of the organelle-specific dynamics associated with the conversion between physiological and pathological states.
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