Fabrication of Biomimetic PCL Scaffold Using Rapid Prototyping for Bone Tissue Engineering

Abstract

We report the fabrication of a porous three-dimensional (3D) scaffold using a 3D plotting system, withapplications in bone tissue engineering. Biomimetic surface coatings of hydroxyapatite were formed using concentratedsimulated body fluid (SBF). The mineralized scaffold had a uniform interconnected porous structure. The apatitethat was formed on the surface of the scaffold was characterized by using energy dispersive spectroscopy (EDS), Xraypowder diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The biological properties of thescaffold were evaluated using WST-1 assays of alkaline phosphatase (ALP) activity and gene expression of D1 mousemesenchymal stem cells. The results show that the biomimetic scaffolds exhibited good apatite-forming properties,with favorable cell proliferation and differentiation for bone formation. Therefore, these biomimetic scaffolds withhydroxyapatite coatings have potential applications in bone regeneration.