gamma-Glutamyl hydrolase modulation and folate influence chemosensitivity of cancer cells to 5-fluorouracil and methotrexate
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, SE (Kim, S-E) | - |
dc.contributor.author | Cole, PD (Cole, P. D.) | - |
dc.contributor.author | Cho, RC (Cho, R. C.) | - |
dc.contributor.author | Ly, A (Ly, A.) | - |
dc.contributor.author | Ishiguro, L (Ishiguro, L.) | - |
dc.contributor.author | Sohn, KJ (Sohn, K-J) | - |
dc.contributor.author | Croxford, R (Croxford, R.) | - |
dc.contributor.author | Kamen, BA (Kamen, B. A.) | - |
dc.contributor.author | Kim, YI (Kim, Y-I) | - |
dc.date.accessioned | 2022-04-19T10:08:49Z | - |
dc.date.available | 2022-04-19T10:08:49Z | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 0007-0920 | - |
dc.identifier.issn | 1532-1827 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/147412 | - |
dc.description.abstract | Background: gamma-Glutamyl hydrolase (GGH) regulates intracellular folate and antifolates for optimal nucleotide biosynthesis and antifolate-induced cytotoxicity, respectively. The modulation of GGH may therefore affect chemosensitivity of cancer cells, and exogenous folate levels may further modify this effect. Methods: We generated a novel model of GGH modulation in human HCT116 and MDA-MB-435 cancer cells and investigated the effect of GGH modulation on chemosensitivity to 5-fluorouracil (5FU) and methotrexate (MTX) at different folate concentrations in vitro and in vivo. Results: Overexpression of GGH significantly decreased chemosensitivity of MDA-MB-435 cells to 5FU and MTX at all folate concentrations as expected. In contrast, in HCT116 cells this predicted effect was observed only at very high folate concentration, and as the folate concentration decreased this effect became null or paradoxically increased. This in vitro observation was confirmed in vivo. Inhibition of GGH significantly increased chemosensitivity of both cancer cells to 5FU at all folate concentrations. Unexpectedly, GGH inhibition significantly decreased chemosensitivity of both cancer cells to MTX at all folate concentrations. In both GGH modulation systems and cell lines, the magnitude of chemosensitivity effect incrementally in | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | gamma-Glutamyl hydrolase modulation and folate influence chemosensitivity of cancer cells to 5-fluorouracil and methotrexate | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1038/bjc.2013.579 | - |
dc.identifier.scopusid | 2-s2.0-84885943348 | - |
dc.identifier.wosid | 000325795000018 | - |
dc.identifier.bibliographicCitation | BRITISH JOURNAL OF CANCER, v.109, no.8, pp 2175 - 2188 | - |
dc.citation.title | BRITISH JOURNAL OF CANCER | - |
dc.citation.volume | 109 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2175 | - |
dc.citation.endPage | 2188 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.