Osteogenic Differentiation of Human Adipose-Derived Stem Cells(hADSCs) on a Dexamethasone Eluting Nanofiber Scaffolds
- Authors
- 이정복; 정성민; 김경준; 조동현; 권일근; 윤인찬; 최귀원; Jun-Kyo Francis Suh; 박재홍; 박용덕; 정종혁; 최경규; 김규태; 최기운; 최용석
- Issue Date
- Mar-2009
- Publisher
- 한국조직공학과 재생의학회
- Keywords
- Adipose-derived stem cells; Dexamethasone eluting; Nanofiber; Osteogenic differentiation
- Citation
- 조직공학과 재생의학, v.6, no.1, pp 371 - 379
- Pages
- 9
- Journal Title
- 조직공학과 재생의학
- Volume
- 6
- Number
- 1
- Start Page
- 371
- End Page
- 379
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148093
- ISSN
- 1738-2696
2212-5469
- Abstract
- Dexamethasone(DEX), a synthetic steroidal anti-inflammatory drug, is an efficient and dependable drug that induces osteogenic differentiation. The aim of this study is to fabricate the DEX loaded PLGA nanofibers by
electrospinning method. Adipose-derived stem cells(ADSCs) were seeded into the nanofiber and the sustained release of DEX from PLGA nanofiber scaffolds promoted their osteogenic differentiation. The properties of DEX
loaded PLGA nanofiber scaffold were characterized by scanning electron microscopy(SEM) and the release kinetics of DEX from PLGA nanofibers in vitro(1 h to 14 days) was evaluated by high performed liquid chromatography(
HPLC). To evaluate the cellular response of the ADSCs seeded onto DEX loaded PLGA nanofiber, we performed F-actin, cytotoxicity, alkaline phosphatase activity, alizarin red S, von Kossa staining and immunocytochemistry assays for osteogenic differentiation. DEX loaded PLGA nanofiber scaffold was observed to have sustained release in vitro during experimental periods. The cytotoxicity test of the DEX loaded PLGA nanofiber
scaffold indicated there was almost no-toxic effects in regards to proliferation and differentiation of adiposederived stem cells(ADSCs) as compared with a control. Alkaline phosphatase activity and alizarin red S were more
significantly increased af
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