Spot arrays on modified glass surfaces for efficient SPOT synthesis and on-chip bioassay of peptides
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Do-Hyun | - |
dc.contributor.author | Shin, Dong-Sik | - |
dc.contributor.author | Lee, Yoon-Sik | - |
dc.date.accessioned | 2022-04-19T11:07:12Z | - |
dc.date.available | 2022-04-19T11:07:12Z | - |
dc.date.issued | 2007-10 | - |
dc.identifier.issn | 1075-2617 | - |
dc.identifier.issn | 1099-1387 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148350 | - |
dc.description.abstract | To make SPOT synthesis of peptides and their assays on glass surfaces more convenient, a simple method for making spot arrays on a slide glass was designed through patterning with a photoresist and perfluorination followed by amination with various silane compounds and polymers. With these spot-arrayed glass surfaces, we could measure the coupling completion of each Fmoc amino acid on the glass surface by direct fluorescence analysis after fluorescence-labeling the amino groups on the surface of each spot. Then we synthesized several types of decapeptides and HPQ-pentapeptides on the spot-arrayed glasses and identified the optimal surface condition for stepwise peptide coupling and on-chip bioassay. After optimizing the surface conditions, we synthesized a model library of biotin-Gly-Ala-P-1-Gly (P-1: one of 19 amino acids) and successfully replicated the well-known a-chymotrypsin subsite specificities through Cy5-streptavidin binding to the remaining biotin on the surface after the enzymatic digestion. Copyright (C) 2007 European Peptide Society and John Wiley & Sons, Ltd. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | JOHN WILEY & SONS LTD | - |
dc.title | Spot arrays on modified glass surfaces for efficient SPOT synthesis and on-chip bioassay of peptides | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1002/psc.884 | - |
dc.identifier.scopusid | 2-s2.0-35348917245 | - |
dc.identifier.wosid | 000250348600001 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PEPTIDE SCIENCE, v.13, no.10, pp 625 - 633 | - |
dc.citation.title | JOURNAL OF PEPTIDE SCIENCE | - |
dc.citation.volume | 13 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 625 | - |
dc.citation.endPage | 633 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/psc.884 | - |
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