Spot arrays on modified glass surfaces for efficient SPOT synthesis and on-chip bioassay of peptides
- Authors
- Kim, Do-Hyun; Shin, Dong-Sik; Lee, Yoon-Sik
- Issue Date
- Oct-2007
- Publisher
- JOHN WILEY & SONS LTD
- Citation
- JOURNAL OF PEPTIDE SCIENCE, v.13, no.10, pp 625 - 633
- Pages
- 9
- Journal Title
- JOURNAL OF PEPTIDE SCIENCE
- Volume
- 13
- Number
- 10
- Start Page
- 625
- End Page
- 633
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148350
- DOI
- 10.1002/psc.884
- ISSN
- 1075-2617
1099-1387
- Abstract
- To make SPOT synthesis of peptides and their assays on glass surfaces more convenient, a simple method for making spot arrays on a slide glass was designed through patterning with a photoresist and perfluorination followed by amination with various silane compounds and polymers. With these spot-arrayed glass surfaces, we could measure the coupling completion of each Fmoc amino acid on the glass surface by direct fluorescence analysis after fluorescence-labeling the amino groups on the surface of each spot. Then we synthesized several types of decapeptides and HPQ-pentapeptides on the spot-arrayed glasses and identified the optimal surface condition for stepwise peptide coupling and on-chip bioassay. After optimizing the surface conditions, we synthesized a model library of biotin-Gly-Ala-P-1-Gly (P-1: one of 19 amino acids) and successfully replicated the well-known a-chymotrypsin subsite specificities through Cy5-streptavidin binding to the remaining biotin on the surface after the enzymatic digestion. Copyright (C) 2007 European Peptide Society and John Wiley & Sons, Ltd.
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