Biphasic role of nuclear factor-kappa B on cell survival and COX-2 expression in SOD1 Tg astrocytes after oxygen glucose deprivation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, YS (Lee, Yong-Sun) | - |
dc.contributor.author | Song, YS (Song, Yun Seon) | - |
dc.contributor.author | Giffard, RG (Giffard, Rona G.) | - |
dc.contributor.author | Chan, PH (Chan, Pak H.) | - |
dc.date.accessioned | 2022-04-19T11:25:03Z | - |
dc.date.available | 2022-04-19T11:25:03Z | - |
dc.date.issued | 2006-08 | - |
dc.identifier.issn | 0271-678X | - |
dc.identifier.issn | 1559-7016 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148563 | - |
dc.description.abstract | In cytoplasm, nuclear factor-kappa B (NF-kappa B) is associated with the inhibitory protein, I kappa B alpha. On activation by H2O2, I kappa B alpha is phosphorylated and degraded, exposing the nuclear localization signals on the NF-kappa B heterodimer. Cyclooxygenase-2 (COX-2), which mediates prostaglandin synthesis during inflammation, is induced by oxidative stress mediated by NF-kappa B. We investigated whether the NF-kappa B signaling pathway affected cell death and COX-2 expression after hypoxia-induced oxidative stress in wild-type (WT) and copper/zinc-superoxide dismutase transgenic (SOD1 Tg) astrocytes. In WT astrocytes, phospho-I kappa B alpha was highly expressed after oxygen-glucose deprivation (OGD) and 2 h of reperfusion, concomitant with the decrease in I kappa B alpha. The NF-kappa B p50 level increased similarly in WT and SOD1 Tg astrocytes (1.2-/1.4-fold) after OGD. Electrophoretic mobility shift assay showed higher DNA-binding activity of NF-kappa B p50 in WT than in SOD1 Tg astrocytes 6 h after 4 h of OGD. The COX-2 level was induced by 2.7- and 1.3-fold after OGD in WT and SOD1 Tg astrocytes, and an antioxidant protected both groups against OGD injury. Superoxide dismutase transgenic cells were 23% more protective against OGD injury than WTs when assessed by lactate dehydrogenase release. Ho | - |
dc.format.extent | 13 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Biphasic role of nuclear factor-kappa B on cell survival and COX-2 expression in SOD1 Tg astrocytes after oxygen glucose deprivation | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.identifier.doi | 10.1038/sj.jcbfm.9600261 | - |
dc.identifier.scopusid | 2-s2.0-33746531703 | - |
dc.identifier.wosid | 000239340700008 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, v.26, no.8, pp 1076 - 1088 | - |
dc.citation.title | JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | - |
dc.citation.volume | 26 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1076 | - |
dc.citation.endPage | 1088 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.url | https://journals.sagepub.com/doi/10.1038/sj.jcbfm.9600261?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.